Method for preparing liposomes
AssigneeUniversity of Greenwich
InventorsSimon Richardson, Benedita Feron
Life of this patent
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Abstract
The invention relates to liposomes, methods of producing liposomes, and methods of loading cell-derived liposomes with cargo molecules. The invention extends to such liposomes per se, and to the use of these liposomes as cellular delivery systems for the delivery of biologically and therapeutically active payload molecules, such as small molecules, RNAi molecules (e.g. siRNA), bioactive proteins, genome editing tools (e.g. Cas9) and drugs into cells for treating a range of disorders. The liposomes may also be used in a range of diagnostic and theranostic applications. The invention extends to pharmaceutical compositions comprising such liposomes, including populations of extracellular vesicles (EV), exosomes and to fusion proteins.
Claims16
A method of preparing a liposome, the method comprising contacting at least one cell with:
- (i) a pore-forming protein, or a pore-forming domain or a variant or fragment thereof;
- and (ii) a shuttle protein attached to a bioactive payload molecule, wherein the pore-forming protein, or the pore-forming domain or the variant or fragment thereof creates a pore through a phospholipid bilayer of the at least one cell, and the shuttle protein interacts with the pore-forming protein, or the pore-forming domain or the variant or fragment thereof, and is internalised into the cell to thereby produce a liposome loaded with a bioactive payload molecule, wherein the method further comprises isolating the liposome from the cell.
Dependencies are inferred from the claim text.