MSC-NTF specific exosomes and use thereof
AssigneeBrainStorm Cell Therapeutics Ltd.
InventorsRevital Aricha, Haggai Kaspi, Jonathan Semo, Yael Gothelf
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Abstract
Provided herein are highly-characterized isolated exosomes, methods to produce such exosomes, and methods for the use of such exosomes in treating diseases such as neurodegenerative diseases.
Claims9
A pharmaceutical composition comprising an isolated exosome population derived from mesenchymal stem cells secreting-neurotrophic factors (MSC-NTF cells), said isolated exosomes comprising an increased quantity of at least one cargo protein, wherein said at least one cargo protein comprises a neurotrophic factor (NTF), compared with the quantity of said at least one cargo protein in an isolated exosome population derived from control non-differentiated MSCs, wherein said NTF comprises a leukemia inhibitory factor (LIF) protein, a vascular endothelial growth factor A (VEGFA) protein, or a growth differentiation factor 15 (GDF15) protein, or any combination thereof,
- wherein said MSC-NTF cells are cultured in a three-dimensional (3D) bioreactor in no shear stress, and propagated in Dulbecco's Modified Eagle's Medium (DMEM) comprising platelet lysate,
- wherein said exosomes are purified by removing the culture media from the three-dimensional (3D) bioreactor, filtered using a 1.2 μM filter and Tangential Flow Filtration (TFF), and further filtered through a 0.22 μM filter.
Dependencies are inferred from the claim text.