Ultrasmall nanoparticles and methods of making, using and analyzing same
AssigneeCornell University
InventorsThomas C. Gardinier, Ferdinand F. E. Kohle, Joshua A. Hinckley, Ulrich B. Wiesner
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Abstract
The present disclosure provides methods of analyzing and/or purifying inorganic nanoparticles that may be functionalized with one or more dye group. Analyzing and/or purifying the inorganic nanoparticles includes utilizing liquid chromatography, such as, for example, high performance liquid chromatography (HPLC). Methods of the present disclosure may be used to determine the location of one or more dye groups on and/or in the inorganic nanoparticles. The present disclosure also provides methods of making inorganic nanoparticles and compositions of inorganic nanoparticles.
Claims17
A method of determining the location of one or more dye on and/or in an inorganic nanoparticle comprising a polyethylene glycol layer, comprising subjecting the inorganic nanoparticle or core-shell nanoparticle to high performance liquid chromatography (HPLC) analysis, wherein the method comprises the steps of:
- (a) depositing the inorganic nanoparticle in an HPLC column comprising an input in fluid communication with a stationary phase in fluid communication with an output in fluid communication with a detector;
- (b) passing a mobile phase through the HPLC column, such that the inorganic nanoparticle elutes from the column and enters the detector, such that the detector generates a signal, wherein the signal indicates the location of the one or more dye on and/or in the inorganic nanoparticle;
- and
- (c) analyzing the signal to determine the location of the one or more dye on and/or in the inorganic nanoparticle.
Dependencies are inferred from the claim text.