Expansion microscopy compatible anchorable HandE staining for histopathology
AssigneeExpansion Technologies
InventorMahender Babu Dewal
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Abstract
A technique for expansion microscopy (ExM) utilizing anchorable derivatives of hematoxylin and eosin (H&E) stained tissue specimens within tissue samples, in order to achieve high resolution detection of biomolecules with precise morphological features of cells and nuclei, which in turn, allows for effective for accurate early stage disease diagnosis including various cancers. Staining with H&E employs anchorable derivatives that can be cross-linked into the ExM hydrogel matrix.
Claims21
An anchorable hematoxylin derivative comprising an anchorable moiety, wherein the hematoxylin is hematein or a hematein analogue.
An anchorable hematoxylin derivative comprising a compound of formula (II)
- wherein Q is O or NH, S is a spacer or a bond, and A is an anchorable moiety capable of being polymerized into a hydrogel matrix.
An anchorable cosin derivative comprising a compound of formula (III) or (IV)
- and wherein Q is O or NH, S is a spacer or a bond, and A is an anchorable moiety capable of being polymerized into a hydrogel matrix.
Dependencies are inferred from the claim text.