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US 12,227,799 B2Granted
Compositions and methods for nucleic acid sequencing
AssigneeSingular Genomics Systems, Inc.
InventorsEli N. Glezer, Mohammad Vatankhah Varnosfaderani, Daan Witters, Vahid Karimkhani
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Abstract
Provided herein are methods and compositions for improved sequencing techniques using, for example, polymeric particles and/or three-dimensional structures.
Claims20
A method of sequencing target polynucleotides in a polymer scaffold comprising polynucleotide primers, wherein said polynucleotide primers are covalently attached to the polymer scaffold, the method comprising:
- (a) amplifying the target polynucleotides to produce discrete amplicon clusters within the polymer scaffold,
- wherein amplifying comprises rolling circle amplification the amplicon clusters are arranged at a plurality of depths;
- and
- (b) sequencing the amplicon clusters, wherein sequencing comprises detecting sequences of signals within the polymer scaffold at a first depth and a second depth.
1 more element
Dependencies are inferred from the claim text.