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US 12,227,784 B2Granted
Enzyme blends and processes for improving the nutritional quality of animal feed
AssigneeNovozymes A/S
InventorsJesper Frickmann, Kendra Stallings, Dan Pettersson, Mads Brøgger Pedersen, Harold Cale Smith
Life of this patent
Estimating the term…
Abstract
The present invention relates to a process for improving the nutritional quality of distillers dried grains (DGS) or distillers dried grains with solubles (DDGS) produced as a co-product of a fermentation product production process, processes for producing fermentation products, as well as enzyme blends used in the processes.
Claims24
A process for producing a fermentation product from starch-containing material comprising the steps of:
- (a) liquefying a starch-containing material with a thermostable alpha-amylase, wherein the starch-containing material is corn;
- (b) saccharifying the liquefied material obtained in step (a) with a glucoamylase and a GH30_8 family xylanase;
- (c) fermenting using a yeast to produce the fermentation product, wherein the fermentation product is fuel ethanol;
- and
- (d) recovering a co-product, wherein the co-product is distillers dried grains (DDG) or distillers dried grains with solubles (DDGS), wherein saccharifying and fermenting are performed simultaneously, and wherein the GH30_8 xylanase is selected from the group consisting of:
- (i) a GH30_8 xylanase having at least 90% amino acid sequence identity to SEQ ID NO:
- 2;
- (ii) a GH30_8 xylanase having at least 90% amino acid sequence identity to SEQ ID NO:
- 3;
- (iii) a GH30_8 xylanase having at least 94% amino acid sequence identity to SEQ ID NO:
- 4;
- (iv) a GH30_8 xylanase having at least 91% amino acid sequence identity to SEQ ID NO:
- 5;
- and
- (v) a GH30_8 xylanase having at least 91% amino acid sequence identity to SEQ ID NO:
- 6.
13 more elements
A process of producing a fermentation product, comprising the following steps:
- (a) saccharifying a starch-containing material at a temperature below the initial gelatinization temperature with an alpha-amylase, a glucoamylase, and a GH30_8 family xylanase, wherein the starch-containing material is corn;
- (b) fermenting using a fermentation organism yeast to produce the fermentation product, wherein the fermentation product is fuel ethanol;
- and
- (c) recovering a co-product, wherein the co-product is distillers dried grains (DDG) or distillers dried grains with solubles (DDGS), wherein saccharifying and fermenting are performed simultaneously, and wherein the GH30_8 xylanase is selected from the group consisting of:
- (i) a GH30_8 xylanase having at least 90% amino acid sequence identity to SEQ ID NO:
- 2;
- (ii) a GH30_8 xylanase having at least 90% amino acid sequence identity to SEQ ID NO:
- 3;
- (iii) a GH30_8 xylanase having at least 94% amino acid sequence identity to SEQ ID NO:
- 4;
- (iv) a GH30_8 xylanase having at least 91% amino acid sequence identity to SEQ ID NO:
- 5;
- and
- (v) a GH30_8 xylanase having at least 91% amino acid sequence identity to SEQ ID NO:
- 6.
12 more elements
Dependencies are inferred from the claim text.