Modified sterol acyltransferases
AssigneeDsm IP Assets B.V.
InventorsChristopher Mark Farrell, Lisa Ann Laprade, Otto Martin Lehmann, Joshua Trueheart
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Abstract
The present invention is related to modified sterol acyltransferase enzymes with improved activity and/or specificity towards acylation of the vitamin D3 precursor 7-dehydrocholesterol (7-DHC) to be used in biotechnological production of vitamin D3. The invention further relates to a yeast strain expressing said modified enzymes and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.
Claims10
A process for production of 7-dehydrocholesterol (7-DHC) comprising enzymatically converting acetyl-CoA into a sterol mix comprising zymosterol and 7-DHC with a host cell, wherein the percentage of 7-DHC in the sterol mix is at least 70%, and wherein the host cell is a recombinant host cell that expresses a modified enzyme selected from EC 2.3.1.26 having sterol acyltransferase activity comprising one or two amino acid substitutions at positions corresponding to residues 592 and 595 in the polypeptide according to SEQ ID NO:1, wherein the substituted amino acid corresponding to residue 592 in the polypeptide according to SEQ ID NO:1 is leucine and the substituted amino acid corresponding to residue 595 in the polypeptide according to SEQ ID NO:1 is aspartic acid.
Dependencies are inferred from the claim text.