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US 12,227,771 B2Granted
Expression systems and methods of use thereof
AssigneeJanssen Pharmaceutica NV
InventorsDavid S. Bredt, Weston Davini, Shenyan Gu, Jose Matta, Min Lei O'Carroll
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Abstract
Expression systems for the expression of an α9α10 nicotinic acetylcholine receptor (nAChR) are described. Also described are methods of using the expression systems to identify agonists, antagonists, or allosteric modulators of an α9α10 nAChR.
Claims6
An expression system for functional expression of α9α10 nAChR comprising a recombinant cell, wherein the recombinant cell is a cell transfected with one or more expression vectors, the one or more expression vectors comprising:
- (i) a first nucleic acid encoding an α9 subunit of an α9α10 nicotinic acetylcholine receptor (nAChR), wherein the α9 subunit of the α9α10 nAChR comprises an amino acid sequence with at least 95% identity to the amino acid sequence of SEQ ID NO:1;
- (ii) a second nucleic acid encoding an α10 subunit of an α9α10 nAChR, wherein the α10 subunit of the α9α10 nAChR comprises an amino acid sequence with at least 95% identity to the amino acid sequence of SEQ ID NO:2;
- and
- (iii) a third nucleic acid encoding Choline O-acetyltransferase (CHAT), wherein the CHAT comprises an amino acid sequence with at least 95% identity to the amino acid sequence of SEQ ID NO:3.
1 more element
Dependencies are inferred from the claim text.