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AssigneesThe University of Chicago, Nationwide Children's Hospital, Inc., The Ohio State University
InventorsJianrong Li, Mark E. Peeples, Chuan He, Stefan Niewiesk, Mijia Lu, Miaoge Xue, Zijie Zhang, Boxuan Zhao
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The current disclosure relates to methods, compositions and kits for detecting modified adenosine in a target RNA molecule. Aspects relate to a method for detecting modified adenosine in a target ribonucleic acid (RNA) comprising contacting the target RNA with an adenosine deaminase enzyme (adenosine deaminase, RNA-specific) to generate a target RNA with deaminated adenosines and sequencing the target RNA with deaminated adenosines; wherein the modified adenosine is detected when the nucleotide sequence includes adenosine within a m6A motif.
An attenuated negative-sense single-stranded RNA virus of the family Pneumoviridae comprising sequence alterations in the viral genome or antigenome corresponding to the G gene encoding one or more N6-methyladenosine (m6A) consensus sites, wherein the alterations result in reduction of m6A modifications of viral mRNA, antigenome or genome.
Dependencies are inferred from the claim text.