Glycosyl hydrolase enzymes in high temperature industrial processes

A mutated recombinant hyperthermophilic Dictyoglomus beta-mannanase (DtManA) enzyme comprising a catalytic domain having an 97% amino acid sequence identity with a native DtManA catalytic domain of SEQ ID NO:

• 3 and a DtManA Carbohydrate Binding Module (CBM) domain that is truncated or mutated to reduce or abolish mannan binding and lacks one or both of a native DtManA CBM sequence NI of SEQ ID NO:
• 22 and a native DtManA CBM sequence N2 of SEQ ID NO:
• 23.

A recombinant mutated hyperthermophilic Dictyoglomus beta-mannanase (DtManA) enzyme comprising a Carbohydrate Binding Module (CBM) domain having an 88% amino acid sequence identity with a native DtManA CBM of SEQ ID NO:

• 21, a catalytic domain having an 97% amino acid sequence identity with a native DtManA catalytic domain of SEQ ID NO:
• 3, and an additional reiterated sequence KLVTPNPSKEAQKL as referenced by SEQ ID NO:
• 25 at a beginning of the catalytic domain.