Methods for selectively expanding cells expressing a TCR with a murine constant region

A method of selectively expanding a number of T cells, the method comprising:

• modifying human T cells to express a TCR, wherein the TCR comprises a murine constant region;
• producing a population of cells comprising a number of the human T cells expressing the TCR and a number of the human T cells not expressing the TCR;
• culturing the population of cells in a first culture,
• wherein the first culture comprises (i) a first population of irradiated feeder cells, (ii) one or more cytokines, and (iii) a first antibody, or an antigen-binding portion thereof, wherein the first antibody, or antigen-binding portion thereof, specifically binds to the murine constant region of the TCR,
• wherein the first culture lacks a second antibody, or an antigen binding portion thereof, which specifically binds to the human CD3 complex, and
• wherein the first culture selectively expands the number of human T cells expressing the TCR over the number of human T cells not expressing the TCR.