Sterile sealing apparatus

A sealing apparatus, comprising:

• an operation chamber;
• a plasma generator having at least one plasma head disposed within the operation chamber and in proximity to a profile comprising a lumen within the operation chamber, wherein the plasma head is configured to apply a plasma treatment to the profile to activate material on an end surface of the profile, within the lumen of the profile, or combinations thereof;
• wherein the profile is manipulated to close the lumen of the profile to aseptically seal the profile;
• and
• a cutting device comprising a blade or laser cutting system and configured to cut a piece of tubing inserted into the operation chamber to form the profile, wherein the plasma treatment is applied to the piece of tubing prior to cutting the piece of tubing to form the profile, wherein the profile comprises a thermoset elastomer.