Apparatus for resolving imaging problems caused by the meniscus
AssigneeAmgen Inc.
InventorsGraham F. Milne, Tony Y. Wang
Life of this patent
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Abstract
A well plate cover includes a base defining a base plane, and a plurality of insertion elements. At least a portion of each of the insertion elements is transparent. Each of the insertion elements is coupled to the base, and extends, in a direction orthogonal to the base plane, from the base to a distal end surface of the insertion element. The distal end surface of each of the insertion elements includes an apex that, when the respective insertion element is inserted into a well of a well plate, extends further into the well than any other portion of the distal end surface. The apex is a point, a line, or a plane having a diameter that is less than one half of a maximum diameter of the distal end surface.
Claims4
A well plate cover comprising:
- a base defining a base plane;
- and
- a plurality of insertion elements, wherein
- at least a portion of each of the insertion elements is transparent,
- each of the insertion elements (i) is coupled to the base, and (iii) extends, in a direction orthogonal to the base plane, from the base to a distal end surface of the insertion element,
- the distal end surface of each of the insertion elements includes an apex that, when the respective insertion element is inserted into a well of a well plate, extends further into the well than any other portion of the distal end surface, and
- the apex is an apex plane having a diameter that is less than one half of a maximum diameter of the distal end surface;
- the apex plane of each of the distal end surfaces forms a circle;
- and
- the distal end surface of each of the insertion elements includes a plurality of planar tiers that (i) form concentric rings around the respective apex plane and (ii) are sized to extend into the well, with each successive planar tier, when progressing outwardly from the apex plane towards a perimeter of the distal end surface, is closer to the base than is the previous planar tier.
A method for imaging a fluid sample in a vessel, the method comprising:
- inserting at least a distal end surface of an insertion element into the fluid sample in the vessel,
- wherein at least a portion of the insertion element is transparent,
- wherein the distal end surface includes an apex that extends further into the vessel than any other portion of the distal end surface, and
- wherein the apex is an apex plane having a diameter that is less than one half of a maximum diameter of the distal end surface;
- wherein:
- the apex plane forms a circle;
- the insertion element includes a proximal end opposite the distal end surface;
- the distal end surface includes a plurality of planar tiers that (i) form concentric rings around the apex plane and (ii) are sized to extend into the well;
- and
- each successive planar tier, when progressing outwardly from the apex plane towards a perimeter of the distal end surface, is closer to the proximal end of the insertion element than is the previous planar tier;
- positioning the vessel in an optical path of an imager;
- illuminating the vessel while the insertion element is inserted into the fluid sample in the vessel;
- and
- capturing, by the imager, an image of the illuminated vessel while the insertion element is inserted into the fluid sample in the vessel.
Dependencies are inferred from the claim text.