Klotho modulation
AssigneeLeonhardt Ventures LLC
InventorHoward J. Leonhardt
Life of this patent
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Abstract
Described is a low voltage, pulsed electrical stimulation device for upregulating expression of klotho, a useful protein, by tissues. Also described are methods of enhancing expression of klotho in cells.
Claims20
A method of treating a cell, the method comprising:
- stimulating the cell to express and/or release Klotho polypeptide by applying a bioelectric signal to the cell,
- wherein the bioelectric signal has a square, biphasic waveform at 50% duty and a frequency selected from the group consisting of 5 Hz, 10 Hz, 20 Hz, 25 Hz, 50 Hz, 75 Hz, 100 Hz, 250 Hz, 500 Hz, 750 Hz, 2,500 Hz, 100,000 Hz, 500,000 Hz, and 1 MHz.
A method of using a bioelectric stimulator programmed to produce one or more bioelectric signals for stimulation of cellular tissue, wherein at least one such bioelectric signal upregulates expression of Klotho by the cellular tissue, the method comprising:
- applying the bioelectric stimulator to target tissue of a subject, and
- actuating the bioelectric stimulator to produce the one or more programmed bioelectric signals,
- wherein the bioelectric stimulator is programmed with at least one bioelectric signal having a square, biphasic waveform at 50% duty and a frequency selected from the group consisting of 5 Hz, 10 Hz, 20 Hz, 25 Hz, 50 Hz, 75 Hz, 100 Hz, 250 Hz, 500 Hz, 750 Hz, 2,500 Hz, 100,000 Hz, 500,000 Hz, and 1 MHz.
A method, of using a bioelectric stimulator programmed to produce one or more bioelectric signals for stimulation of cellular tissue, wherein at least one such bioelectric signal downregulates expression of Klotho by the cellular tissue, the method comprising:
- applying the bioelectric stimulator to target tissue of a subject, and
- actuating the bioelectric stimulator to produce the one or more programmed bioelectric signals,
- wherein the bioelectric stimulator is programmed with at least one bioelectric signal having a square, biphasic waveform at 50% duty and having a frequency selected from the group consisting of 25,000 Hz, 50,000 Hz, 750,000 Hz, and 1 MHz.
Dependencies are inferred from the claim text.