Stable nanolipoprotein particles and related compositions methods and systems
AssigneeLawrence Livermore National Security, LLC
InventorsCraig D. Blanchette, Nicholas Fischer, Sean Fitzpatrick Gilmore, Amy Rasley, Paul Henderson
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Abstract
Nanolipoprotein particles having at least a scaffold protein component and a membrane lipid component and related compositions, methods and systems are described. The membrane lipid component includes at least one or more membrane forming lipids, one or more polymerized lipids and/or one or more polymerizable lipids.
Claims36
A nanolipoprotein particle comprising:
- a membrane forming lipid, a polymerized lipid and a scaffold protein,
- the membrane forming lipid and the polymerized lipid arranged in a membrane forming lipid discoidal bilayer stabilized by the scaffold protein and by the polymerized lipid,
- wherein the polymerized lipid is formed by crosslinking polymerizable lipids within the membrane forming lipid discoidal bilayer in a molar concentration of about 5 to about 40 mol %.
A nanolipoprotein particle comprising a cross-linked membrane lipid bilayer confined in a discoidal configuration by a scaffold protein, the cross-linked membrane lipid bilayer comprising one or more polymerized lipids and one or more membrane forming lipids.
A nanolipoprotein particle comprising:
- a membrane forming lipid, a polymerized lipid and a scaffold protein,
- the membrane forming lipid and the polymerized lipid arranged in a membrane forming lipid discoidal bilayer stabilized by the scaffold protein and by the polymerized lipid,
- wherein the polymerized lipid is formed by crosslinking polymerizable lipids, and wherein the polymerized lipid is in a molar concentration of about 10 to about 30 mol % and a total lipid to scaffold protein molar percent ratio ranges from 20:1 to 240:1.
Dependencies are inferred from the claim text.