# US 12,224,171 B2 · Electrospray probe > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,224,171 B2 - Document ID: US12224171B2 - Status: Granted - Kind: B2 - Application number: 17/440,131 - Filed: 2020-05-29 - Published: 2022-05-19 - Granted: 2025-02-11 - Earliest claimed: 2019-05-31 - Assignees: DH Technologies Development Pte. Ltd. - Applicants: DH Technologies Development PTE. LTD. - Inventors: Leigh Bedford; Goran Ristic; Bradley B. Schneider - Web page: https://patentgrep.com/patent/US12224171B2 ## CPC - H01J 49/167: {Capillaries and nozzles specially adapted therefor; (electrostatic spraying per se B05B5/00)} - G01N 30/7266: {by electric field, e.g. electrospray} - H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract An electrospray probe for use in an electrospray ion source is disclosed, which comprises a cannula extending from a proximal end having an inlet aperture for receiving a liquid sample containing at least one analyte to a discharge emitter end having an outlet aperture through which charged liquid droplets containing ions of said analyte are discharged, and an electrically conductive coating covering at least a portion of an external surface and at least a portion of an internal surface of said emitter end. ## Claims ### Claim 1 An electrospray emitter for use in an electrospray ion source, comprising: a cannula extending from a proximal end having an inlet aperture for receiving a liquid sample containing at least one analyte to a discharge emitter end having an outlet aperture through which charged liquid droplets containing ions of said analyte are discharged, and an electrically conductive coating covering at least a portion of an external surface and at least a portion of an internal surface of said emitter end, wherein the at least the portion of the external surface of said emitter end and the coating covering the external surface of the emitter end include a tapered tip extending to the outlet aperture of the emitter end. ### Claim 2 (depends on 1) The electrospray emitter of claim 1, wherein the tapered tip, of the external surface and the coating covering the external surface, comprises a rounded tapering tip. ### Claim 3 (depends on 2) The electrospray emitter of claim 2, wherein said rounded tapering tip has a curved radius in a range of about 20 microns to about 200 microns. ### Claim 4 (depends on 1) The electrospray emitter of claim 1, wherein said proximal end of the cannula is configured for coupling to a liquid chromatography (LC) column for receiving said liquid sample. ### Claim 5 (depends on 1) The electrospray emitter of claim 1, further comprising a stationary phase of an LC column disposed in a portion of said cannula upstream of said outlet aperture. ### Claim 6 (depends on 1) The electrospray emitter of claim 1, wherein said electrically conductive coating comprises any of single and multilayer coating of a metal or an alloy. ### Claim 7 (depends on 1) The electrospray emitter of claim 1, wherein said electrically conductive coating has a thickness in a range of about 5 Angstroms to about 5 microns. ### Claim 8 (depends on 1) The electrospray emitter of claim 1, wherein said cannula is configured to accommodate a flow rate of said liquid sample in a range of about 1 nL/min to about 5 mL/min. ### Claim 9 (depends on 8) The electrospray emitter of claim 8, wherein said outlet aperture has a diameter in a range of about 1 micron to about 150 microns. ### Claim 10 (depends on 1) The electrospray emitter of claim 1, wherein said outlet aperture of the cannula has a diameter in a range of about 2 to about 40 microns and is configured for nanoflow electrospray ionization at flow rates less than about 2 microliters/min. ### Claim 11 A mass spectrometer system, comprising: an ion source having an electrospray probe for generating ions, a curtain plate having an orifice for receiving at least a portion of said ions, one or more mass analyzers disposed downstream of said orifice of the curtain plate, wherein said electrospray probe comprises: a cannula extending from a proximal end having an inlet aperture for receiving a liquid sample containing at least one analyte to a discharge emitter end having an outlet aperture through which charged liquid droplets containing ions of said analyte are discharged, and an electrically conductive coating covering at least a portion of an external surface and at least a portion of an internal surface of said emitter end, wherein the at least the portion of the external surface of said emitter end and the coating covering the external surface of the emitter end include a tapered tip extending to the outlet aperture of the emitter end. ### Claim 12 (depends on 11) The mass spectrometer of claim 11, wherein the tapered tip, of the external surface and the coating covering the external surface, comprises a rounded tapering tip. ### Claim 13 (depends on 12) The mass spectrometer of claim 12, wherein said rounded tapering tip has a curved radius in a range of about 20 microns to about 200 microns. ### Claim 14 (depends on 11) The mass spectrometer of claim 11, wherein said proximal end of the cannula is configured for coupling to a liquid chromatography (LC) column for receiving said liquid sample; and optionally the mass spectrometer further comprising a stationary phase of an LC column disposed in a portion of said cannula upstream of said outlet aperture. ### Claim 15 (depends on 11) The mass spectrometer of claim 11, wherein said electrically conductive coating comprises any of a single and a multilayer coating of a metal or an alloy; and optionally wherein said electrically conductive coating has a thickness in a range of about 50 Angstroms to about 5 microns. ### Claim 16 (depends on 11) The mass spectrometer of claim 11, wherein said cannula is configured to accommodate a flow rate of said liquid sample in a range of about 1 nL/min to about 5 mL/min. ### Claim 17 (depends on 16) The mass spectrometer of claim 16, wherein said outlet aperture has a diameter in a range of about 1 micron to about 150 microns. ### Claim 18 (depends on 11) The mass spectrometer of claim 11, wherein at least one of said mass analyzers comprises a quadrupole mass analyzer; optionally, the mass spectrometer further comprising a detector disposed downstream of said one or more mass analyzers for detecting ions and generating ion detection signals; and optionally the mass spectrometer further comprising an analyzer for receiving said ion detection signals and generating a mass spectrum of said ions. ### Claim 19 A process for ionizing a sample, comprising: providing an electrospray probe comprising a cannula extending from a proximal end having an inlet aperture for receiving a liquid sample containing at least one analyte to a discharge emitter end having an outlet aperture through which charged liquid droplets containing ions of said analyte are discharged, and an electrically conductive coating covering at least a portion of an external surface and at least a portion of an internal surface of said emitter end, wherein the at least the portion of the external surface of said emitter end and the coating covering the external surface of the emitter end include a tapered tip extending to the outlet aperture of the emitter end, maintaining said conductive coating at an electric potential difference relative to a counter electrode, introducing a liquid sample via said inlet aperture into said cannula so as to provide a flow of the liquid sample from said inlet aperture to said outlet aperture, wherein said liquid sample is discharged via said outlet aperture as a plurality of charged liquid droplets containing ions of said analyte. ### Claim 20 (depends on 19) The process of claim 19, wherein the tapered tip, of the external surface and the coating covering the external surface, has a rounded tapering tip.