# US 12,222,486 B2 · Light source apparatus, microscopic device, optical detection device and optical detection method

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- Number: US 12,222,486 B2
- Document ID: US12222486B2
- Status: Granted
- Kind: B2
- Application number: 18/269,933
- Filed: 2021-02-19
- Published: 2024-01-11
- Granted: 2025-02-11
- Earliest claimed: 2020-12-28
- Assignees: Bioaces (Shanghai) Life Science Co., Ltd
- Applicants: Bioaces (Shanghai) Life Science Co., Ltd
- Inventors: Ziyin Feng; Minbiao Ji
- Web page: https://patentgrep.com/patent/US12222486B2

## CPC

- G02B 21/06: Means for illuminating specimens
- G01J 3/44: Raman spectrometry; Scattering spectrometry {; Fluorescence spectrometry}
- G01N 21/01: Arrangements or apparatus for facilitating the optical investigation
- G01N 21/63: optically excited
- G01N 21/64: Fluorescence; Phosphorescence
- G02B 13/00: Optical objectives specially designed for the purposes specified below (with variable magnification {in general} G02B15/00)
- G02B 21/02: Objectives
- G02B 21/125: {affording both dark- and bright-field illumination}
- G02B 21/16: adapted for ultraviolet illumination {; Fluorescence microscopes (G02B21/0076 takes precedence)}
- G02B 27/09: Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for {(adapting the beam shape of a laser diode G02B19/0052; adapting the beam shape of an LED G02B19/0061; coupling into light guides using intermediate optical elements G02B6/4204; beam shaping specially adapted for lasers H01S3/005)}
- G02B 27/1006: {for splitting or combining different wavelengths (G02B27/1086, G02B27/141 take precedence)}
- G02B 27/141: {using dichroic mirrors}
- G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G01J: MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY (light sources F21, H01J, H01K, H05B; investigating properties of materials by optical means G01N)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present disclosure relates to a light source apparatus, a microscopic device, an optical detection device, and an optical detection method. The light source apparatus comprises: an illumination light source configured to generate illumination light; and a diaphragm disposed on an emergent light path of the illumination light source, comprising: a light shielding screen configured to block part of the illumination light; a first light-transmitting portion provided on the light shielding screen and covering a center of the diaphragm, the first light-transmitting portion being configured to make part of the illumination light transmit therethrough to form bright-field illumination; and a second light-transmitting portion provided on the light shielding screen and located at a periphery of the first light-transmitting portion, the second light-transmitting portion being configured to make part of the illumination light transmit therethrough to form dark-field illumination.

## Claims

### Claim 1

A microscopic device, comprising a light source apparatus for optical detection and an objective lens disposed opposite to the light source apparatus with respect to a sample position, wherein the light source apparatus comprises:
a first light source assembly configured to generate a first emergent light propagating in a first direction;
a second light source assembly configured to generate a second emergent light propagating in a second direction, wherein the second direction and the first direction intersect with each other, one of the first emergent light and the second emergent light is a light for illumination, another one of the first emergent light and the second emergent light is a light for excitation, one of the first and second light source assemblies that is configured to generate the light for illumination is an illumination light source assembly, and another one of the first and second light source assemblies that is configured to generate the light for excitation is an excitation light source assembly, the light for illumination and the light for excitation are configured to be switched on at different time;
a first dichroscope disposed at a location where the first direction intersects the second direction, and configured to make at least part of the first emergent light that is in a first band of wavelength transmit to continue propagating in the first direction, and to reflect at least part of the second emergent light that is in a second band of wavelength to propagate in the first direction, wherein the first band of wavelength and the second band of wavelength are different from each other;
wherein the illumination light source assembly comprises:
an illumination light source; and
a diaphragm disposed between the illumination light source and the first dichroscope, and configured to block part of the light for illumination to form composite illumination combined with bright-field illumination and dark-field illumination simultaneously, wherein the diaphragm comprises a light shielding screen, one light-passing hole provided at a central location of the light shielding screen to form the bright-field illumination, and a plurality of light-passing holes or light-passing slits provided on the light shielding screen and distributed around the light-passing hole at the central location to form the dark-field illumination;
wherein a distance R1 between an outer edge of the light-passing hole provided at the central location of the light shielding screen and a center of the diaphragm, a distance l between the diaphragm and the sample position, and a numerical aperture n of the objective lens satisfy following relationship: R1≤l·tg[arcsin(n)/3], and a distance R2 between an inner edge of the light-passing hole or light-passing slit distributed around the light-passing hole at the central location and the center of the diaphragm, the distance 1 between the diaphragm and the sample position, and the numerical aperture n of the objective lens satisfy following relationship:
R2>l·tg[arcsin(n)].

### Claim 2 (depends on 1)

The microscopic device according to claim 1, wherein a minimum wavelength of the light for illumination is greater than a maximum wavelength of the light for excitation.

### Claim 3 (depends on 1)

The microscopic device according to claim 1, wherein the illumination light source assembly further comprises:
a second lens disposed between the illumination light source and the diaphragm, and configured to collimate the light for illumination.

### Claim 4 (depends on 1)

The microscopic device according to claim 1, wherein the illumination light source comprises at least one of a thermal radiation light source or a light emitting diode.

### Claim 5 (depends on 1)

The microscopic device according to claim 1, wherein the diaphragm comprises an adjustable diaphragm configured to enable part of the light for illumination that passes through the diaphragm to be changed.

### Claim 6 (depends on 1)

The microscopic device according to claim 1, wherein the excitation light source assembly comprises:
an excitation light source; and
a first filter disposed between the excitation light source and the first dichroscope, and configured to filter light generated by the excitation light source.

### Claim 7 (depends on 6)

The microscopic device according to claim 6, wherein the excitation light source assembly further comprises:
a third lens disposed between the excitation light source and the first filter, and configured to collimate the light generated by the excitation light source.

### Claim 8 (depends on 6)

The microscopic device according to claim 6, wherein the excitation light source comprises at least one of a light emitting diode or a laser.

### Claim 9 (depends on 6)

The microscopic device according to claim 6, wherein the first filter comprises a bandpass filter.

### Claim 10 (depends on 1)

The microscopic device according to claim 1, wherein the first direction and the second direction are perpendicular to each other.

### Claim 11 (depends on 1)

The microscopic device according to claim 1, wherein incident angles of the first emergent light and the second emergent light with respect to the first dichroscope are both 45 degrees.

### Claim 12 (depends on 1)

The microscopic device according to claim 1, wherein the microscopic device further comprises:
a fourth lens disposed between the first dichroscope and the sample position, and configured to converge at least one of the first emergent light or the second emergent light to the sample position.