# US 12,222,340 B2 · Preparative liquid chromatograph

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- Number: US 12,222,340 B2
- Document ID: US12222340B2
- Status: Granted
- Kind: B2
- Application number: 17/676,164
- Filed: 2022-02-20
- Published: 2022-09-08
- Granted: 2025-02-11
- Earliest claimed: 2021-03-02
- Assignees: Shimadzu Corporation
- Applicants: Shimadzu Corporation
- Inventors: Shiori Ueda; Tomohiro Shagawa; Soichiro Tamaoki; Tomoyuki Yamazaki; Shohei Sato
- Web page: https://patentgrep.com/patent/US12222340B2

## CPC

- G01N 30/82: Automatic means therefor
- B01D 15/247: {Fraction collectors}
- G01N 30/16: Injection (G01N30/24 takes precedence)
- G01N 30/466: {with separation columns in parallel}
- G01N 30/8679: {Target compound analysis, i.e. whereby a limited number of peaks is analysed}
- G01N 2030/027: {Liquid chromatography}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)

## Abstract

A preparative liquid chromatograph for separating and extracting components in a sample, the preparative liquid chromatograph including a separation channel (2) through which a mobile phase flows, an injector (6) that injects the sample into the mobile phase, a separation column (8) for separating components in the sample injected into the mobile phase, the separation column (8) being provided downstream of the injector (6) on the separation channel (2), a detector (10) for detecting peaks of the components separated by the separation column (8), a fraction collector (12) configured to collect an eluate from the separation column (8) into a plurality of collection containers (20:22) at a downstream of the detector (10) while fractionating the eluate, and a controller (14) for controlling operation of the fraction collector (12), wherein the fraction collector (12) is provided with peak collection containers (20) and waste liquid collection containers (22), each peak collection container (20) is used for collecting a peak portion including a collection target peak detected by the detector (10) in the eluate, and each waste liquid collection container (22) is used for collecting a waste liquid portion not including the collection target peak in the eluate, and the controller (14) is configured to collect the peak portion including the collection target peak detected by the detector (10) in the eluate in the peak collection container (20), and to collect at least a part of the waste liquid portion in the waste liquid collection container (22).

## Claims

### Claim 1

A preparative liquid chromatograph for separating and extracting components in a sample, the preparative liquid chromatograph comprising: a separation channel through which a mobile phase flows;
an injector that injects the sample into the mobile phase;
a separation column for separating the components in the sample injected into the mobile phase, the separation column being provided downstream of the injector on the separation channel;
a detector for detecting peaks of the components separated by the separation column;
a fraction collector configured to collect an eluate from the separation column into a plurality of collection containers at a downstream of the detector while fractionating the eluate;
wherein the fraction collector is provided with both a set of peak collection containers and a set of waste liquid collection containers, each peak collection container configured to collect a peak portion including a collection target peak detected by the detector in the eluate, and each waste liquid collection container configured to collect a waste liquid portion not including the collection target peak in the eluate;
a controller configured for controlling operation of the fraction collector;
such that the controller is configured to collect the peak portion including the collection target peak detected by the detector in the eluate in the peak collection container, and to collect at least a part of the waste liquid portion in the waste liquid collection container.

### Claim 2 (depends on 1)

The preparative liquid chromatograph according to claim 1, wherein the controller is configured to require a user to designate, on a chromatogram of the sample acquired in advance, at least one collection target peak to be collected in the peak collection container, and is configured to collect at least one peak portion each including the at least one collection target peak designated by the user in the peak collection container and to collect waste liquid portions before and after the at least one peak portion in the waste liquid collection containers.

### Claim 3 (depends on 2)

The preparative liquid chromatograph according to claim 2, wherein the controller is configured to require a user to designate an amount of each of the waste liquid portions to be collected before and after the at least one peak portion in the waste liquid collection container.

### Claim 4 (depends on 1)

The preparative liquid chromatograph according to claim 1, wherein the controller is configured to collect all of the waste liquid portion in the waste liquid collection container after separation and extraction, which is started by injecting a sample into the mobile phase, wherein sample injection is started when the separation and extraction are completed.