Electrical impedance monitoring device, system and method for locomotion behaviors of Caenorhabditis elegans

An electrical impedance monitoring device for locomotion behaviors of C. elegans, comprising a circuit board, a microfluidic channel layer and a gas channel layer, wherein the microfluidic channel layer and the gas channel layer form a double-layer structure fixed to the circuit board;

• the circuit board, as a substrate of the electrical impedance monitoring device, is used for carrying the double-layer structure formed by bonding of the microfluidic channel layer and the gas channel layer;
• an electrode array is arranged on the circuit board and used for realizing current excitation and electrical impedance monitoring:
• the microfluidic channel layer comprises a fluid inlet, a food loading channel inlet, a C. elegans loading channel, a culture and monitoring chamber, a C. elegans transition channel, a food loading channel, an impurity discharge channel and a fluid outlet, wherein the fluid inlet, the C. elegans loading channel, the culture and monitoring chamber, the impurity discharge channel and the fluid outlet are connected in sequence;
• the gas channel layer comprises the fluid inlet, the food loading channel inlet, a first gas inlet, a second gas inlet, a third gas inlet, a fourth gas inlet, a first membrane valve, a second membrane valve, a third membrane valve, a fourth membrane valve, a fifth membrane valve, a sixth membrane valve and the fluid outlet;
• wherein, the first gas inlet, the second gas inlet, the third gas inlet and the fourth gas inlet are used for providing a gas pressure for the first membrane valve, the second membrane valve, the third membrane valve, the fourth membrane valve, the fifth membrane valve and the sixth membrane valve, and when the gas pressure increases, the membrane valves will squeeze an upper wall of the microfluidic channel layer to control the microfluidic channel layer to be opened or closed;
• the food loading channel is connected to the culture and monitoring chamber and is controlled by the second membrane valve to be opened or closed;
• the C. elegans transition channel is arranged an outlet of the culture and monitoring chamber and returns to the culture and monitoring chamber, and C. elegans enters the C. elegans transition channel from the culture and monitoring chamber under the control of fluid, the third membrane valve, the fourth membrane valve, the fifth membrane valve and the sixth membrane valve, and then returns to the culture and monitoring chamber.