# US 12,222,289 B2 · Reaction processor

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- Number: US 12,222,289 B2
- Document ID: US12222289B2
- Status: Granted
- Kind: B2
- Application number: 17/124,655
- Filed: 2020-12-17
- Published: 2021-04-08
- Granted: 2025-02-11
- Earliest claimed: 2018-07-06
- Assignees: Go!Foton, Inc.
- Applicants: Go!Foton, Inc.
- Inventors: Shuya Ogi; Takashi Fukuzawa
- Web page: https://patentgrep.com/patent/US12222289B2

## CPC

- G01N 21/645: {Specially adapted constructive features of fluorimeters}
- G01N 21/6486: {Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence)}
- B01L 7/525: {with physical movement of samples between temperature zones}
- C12M 1/00: Apparatus for enzymology or microbiology
- C12Q 1/686: Polymerase chain reaction [PCR]
- G01N 37/00: Details not covered by any other group of this subclass
- B01L 2200/0689: Sealing
- B01L 2200/147: Employing temperature sensors
- B01L 2300/0654: Lenses; Optical fibres
- B01L 2300/0883: Serpentine channels
- B01L 2300/168: Specific optical properties, e.g. reflective coatings
- B01L 2300/1822: using Peltier elements
- B01L 2300/1827: using resistive heater
- G01N 21/0332: {with temperature control (control of temperature G05D23/00; cryostats F17C3/08)}
- G01N 2021/174: {either absorption-reflection or emission-fluorescence}
- G01N 2021/6419: {Excitation at two or more wavelengths}
- G01N 2021/6421: {Measuring at two or more wavelengths}
- G01N 2021/6482: {Sample cells, cuvettes}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

A reaction processor includes: a reaction processing vessel; a first optical head including a first objective lens OB1 for irradiating a sample with first excitation light and collecting first fluorescence generated from the sample; a second optical head including a second objective lens for irradiating a sample with second excitation light and collecting second fluorescence generated from the sample; and a holding member holding the first optical head and the second optical head. The wavelength range of the first fluorescence and the wavelength range of the second excitation light at least partially overlap with each other. A distance between the optical axis of the first objective lens and the optical axis of the second objective lens satisfies 2·P0+2·P1+4·P2+4·P3<P, P0=L·NA/√(1−NA2), P1=t1·NA/√(n12−NA2), P2=t2·NA/√(1−NA2), and P3=t3·NA/√(n32−NA2).

## Claims

### Claim 1

A reaction processor comprising:
a reaction processing vessel including a substrate having a first main surface with a channel through which a sample moves, and a channel sealing film disposed on the first main surface so as to seal the channel;
a first optical head including a first objective lens structured to irradiate a sample in the channel with first excitation light and to collect first fluorescence generated from the sample by irradiation with the first excitation light;
a second optical head including a second objective lens structured to irradiate a sample in the channel with second excitation light and to collect second fluorescence generated from the sample by irradiation with the second excitation light; and
a holding member holding the first optical head and the second optical head, wherein
the first optical head and the second optical head are disposed side by side in a longitudinal direction of the channel,
a wavelength range of the first fluorescence and a wavelength range of the second excitation light at least partially overlap with each other, and
the holding member has a surface that faces the channel sealing film,
the surface of the holding member that faces the channel sealing film is flush with excitation light emitting side end surfaces of the first objective lens and the second objective lens,
a distance P between an optical axis of the first objective lens and an optical axis of the second objective lens satisfies the following formula:
2·P0+2·P1+4·P2+4·P3<P,
P0=L·NA/√(1−NA2),
P1=t1·NA/√(n12−NA2),
P2=t2·NA/√(1−NA2), and
P3=t3·NA/√(n32−NA2)
(in which L represents a distance from the holding member to the channel sealing film, t1 represents a thickness of the channel sealing film, t2 represents a depth of the channel, t3 represents a thickness from a bottom of the channel to a second main surface of the substrate, NA represents a numerical aperture of the first objective lens and the second objective lens, n1 represents a refractive index of the channel sealing film, and n3 represents a refractive index of the substrate).

### Claim 2 (depends on 1)

The reaction processor according to claim 1, wherein the distance P further satisfies 1.1×(2·P0+2·P1+4·P2+4·P3)≤P, and more preferably satisfies 1.2×(2·P0+2·P1+4·P2+4·P3)≤P.

### Claim 3 (depends on 1)

The reaction processor according to claim 1, wherein the distance P satisfies P≤ S−2× ΔS (in which S represents a length of a linear portion of the channel in which the first optical head and the second optical head are disposed, and ΔS represents 1 mm).

### Claim 4 (depends on 1)

The reaction processor according to claim 1, further comprising a light absorbing layer structured to absorb excitation light between the bottom of the channel and the second main surface in the substrate.