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AssigneeJiangnan University
InventorsLong Liu, Jian Chen, Guocheng Du, Yaokang Wu, Taichi Chen, Jianghua Li
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The present invention provides a method for promoting N-acetylglucosamine synthesis by using the GlcN6P responsive element. In the present invention, Bacillus subtilis BSGNY-Pveg-glmS-P43-GNA1 is used as a starting strain, in which a CRISPRi system regulated by GlcN6P responsive element is integrated into the genome to dynamically weaken the N-acetylglucosamine synthesis competitive pathway; a GlcN6P responsive promoter is used to regulate the expression of GNA1 on the plasmid to dynamically regulate the N-acetylglucosamine synthesis pathway; and the key gene alsSD involved in the acetoin synthesis pathway is knocked out. During fed-batch fermentation with this strain in a 15 L fermenter, the production of N-acetylglucosamine reaches 131.6 g/L and no by-product acetoin is accumulated, which lays a foundation for the production of GlcNAc by industrial fermentation.
A method for promoting N-acetylglucosamine synthesis, comprising controlling the expression of glucosamine 6-phosphate N-acetyltransferase GNA4 by GNA1 by using a GlcN6P responsive element to dynamically regulate the N-acetylglucosamine synthesis pathway;
A recombinant Bacillus subtilis, wherein a GlcN6P responsive element is used to control the expression of glucosamine 6-phosphate N-acetyltransferase GNA1 to dynamically regulate the N-acetylglucosamine synthesis pathway;
Dependencies are inferred from the claim text.