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US 12,221,613 B2Granted
Approaches to dramatically increase rice productivity
AssigneeAcademia Sinica
InventorsSu-Ying Yeh, Hsin-Hung Lin, Maurice S. B. Ku, Wen-Hsiung Li
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AssigneeAcademia Sinica
InventorsSu-Ying Yeh, Hsin-Hung Lin, Maurice S. B. Ku, Wen-Hsiung Li
Estimating the term…
A transgenic rice plant containing in its genome a recombinant DNA construct that includes a first nucleic acid having a sequence of a first Golden 2-like transcription factor (GLK) gene operably linked to its natural promoter and 5′ untranslated region (5′UTR), and a second nucleic acid having a sequence of a second GLK gene operably linked to its natural promoter and 5′UTR, the second GLK gene being distinct from the first GLK gene. The first GLK gene and the second GLK gene are both from a C4 plant and the transgenic rice plant exhibits a 65-106% increase in shoot biomass and a 50-95% increase in grain yield, as compared to an untransformed wild-type rice plant. Also provided is a method for producing the transgenic rice plant and a recombinant DNA construct that can be used in the method.
A transgenic rice plant, comprising in its genome a recombinant DNA construct that contains a first nucleic acid having a sequence of a first Golden 2-like transcription factor (GLK) gene operably linked to its natural promoter and 5′ untranslated region (5′UTR), and a second nucleic acid having a sequence of a second GLK gene operably linked to its natural promoter and 5′UTR, wherein the first GLK gene is Zea mays GLK1, the second GLK gene is Zea mays GLK2, both the first GLK gene and the second GLK gene are expressed in the transgenic rice plant, and the transgenic rice plant exhibits at least a 50% increase in shoot biomass and grain yield, as compared to an untransformed wild-type rice plant.
A method of producing a transgenic rice plant, the method comprising:
A recombinant DNA construct, comprising a first nucleic acid sequence that includes a first Golden 2-like transcription factor (GLK) gene operably linked to its natural promoter and 5′UTR, and a second nucleic acid sequence that includes a second GLK gene operably linked to its natural promoter and 5′UTR, wherein the first GLK gene is Zea mays GLK1 and the second GLK gene is Zea mays GLK2.
Dependencies are inferred from the claim text.