Plants having increased oil quality
AssigneesRegents of the University of Minnesota, The Board of Trustees of Illinois State University
InventorsMichael David Marks, Ratan Chopra, Nicole Folstad, Donald L. Wyse, John C. Sedbrook
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Abstract
This document relates to methods and materials for generating oilseed (e.g., pennycress) plants that have oil with reduced levels of polyunsaturated fatty acids (PUFAs) and/or increased levels of oleic acid. For example, oilseed plants having reduced expression levels of one or more polypeptides involved in fatty acid biosynthesis (e.g., fatty acid desaturase 2 (FAD2) and reduced oleate desaturation 1 (ROD1)), as well as methods and materials for making and using such oilseed plants are provided.
Claims14
A pennycress plant having reduced levels of polyunsaturated fatty acids (PUFAs) and/or increased levels of oleic acid, said pennycress plant comprising:
- a genome comprising a modified reduced oleate desaturation 1 (ROD1) gene, wherein the modified ROD1 gene comprises a loss-of-function modification effective to knock-out expression levels of ROD1 polypeptides and/or ROD1 polypeptide activity;
- wherein an unmodified coding sequence of the ROD1 gene encodes a ROD1 polypeptide having the amino acid sequence shown in SEQ ID NO:10;
- and
- wherein the modification in the ROD1 gene is effective to reduce levels of PUFAs and/or increase levels of oleic acid in the plant compared to a corresponding pennycress plant lacking the modification in the ROD1 gene.
Dependencies are inferred from the claim text.