Skin chip, method for manufacturing skin chip and cell skin chip, method for manufacturing skin chip and cell

A method for manufacturing a skin chip, the method comprising:

• forming first and second polydimethylsiloxane (PDMS) layers disposed on both surfaces of a porous membrane, each of the first and second PDMS layers having a microfluidic channel through which a culture medium is transferred to the both surfaces of the porous membrane, wherein the microfluidic channel of the first PDMS layer is a first channel and the microfluidic channel of the second PDMS layer is a second channel;
• forming first and second microelectrode array (MEA) substrate layers disposed on outer surfaces of the first and second PDMS layers, respectively, the first and second MEA substrate layers having metal electrodes for measuring cellular resistance arranged at positions corresponding to the microfluidic channels;
• and
• forming first and second poly (methyl methacrylate) (PMMA) layers disposed on outer surfaces of the first and second MEA substrate layers, respectively,
• wherein the first channel is formed on the first PDMS layer and the second channel is formed on the second PDMS layer, and the first and second channels are formed perpendicular to each other,
• wherein the first PDMS layer is attached to an upper surface of the porous membrane and has a through-hole through which the first channel and a pressurizing means are penetrated,
• wherein the second PDMS layer is attached to a lower surface of the porous membrane and has a through-hole through which the second channel and the pressurizing means are penetrated,
• wherein the metal electrodes include a first metal electrode and a second metal electrode,
• wherein the first MEA substrate layer is attached to an upper surface of the first PDMS layer to form a through-hole through which the first metal electrode and the pressurizing means are penetrated,
• wherein the first metal electrode is formed in a first region corresponding to the first channel,
• wherein the second MEA substrate layer is attached to a lower surface of the first PDMS layer to form a through-hole through which the second metal electrode and the pressurizing means are penetrated, and
• wherein the second metal electrode is formed in a second region corresponding to the second channel.