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US 12,220,235 B2Granted
Analyte sensors and sensing methods for dual detection of glucose and ethanol
AssigneeAbbott Diabetes Care Inc.
InventorsTianmei Ouyang, Benjamin J. Feldman, Hyun Cho, Lam N. Tran, Stephen Oja, Namvar Kiaie
Life of this patent
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Abstract
Multiple enzymes may be present in one or more active areas of an electrochemical analyte sensor for detecting one or more different analytes. In particular, an analyte sensor may comprise a sensor tail configured for insertion into a tissue and one or more working electrodes having a glucose-responsive active area and an ethanol-responsive active area to detect glucose and ethanol in vivo.
Claims12
An analyte sensor comprising:
- a) a working electrode;
- b) a glucose-responsive active area disposed upon a surface of the working electrode, the glucose-responsive active area comprising a glucose-responsive active enzyme capable of generating a first signal at the working electrode proportional to a glucose concentration;
- c) an ethanol-responsive active area comprising:
- i) a first active area disposed directly upon the surface of the working electrode spaced apart from the glucose-responsive active area, the first active area comprising xanthine oxidase and an electron transfer agent, in amounts sufficient to react with acetaldehyde to generate a second signal at the working electrode proportional to an alcohol concentration;
- and
- ii) a second active area isolated from the working electrode, the second active area comprising glucose oxidase and catalase in amounts sufficient to form acetaldehyde upon exposure to ethanol;
- d) a first membrane permeable to acetaldehyde disposed upon the first active area;
- and
- e) a second membrane permeable to glucose and ethanol disposed upon the glucose-responsive active area and the second active area,
- wherein the second active area is disposed directly upon the first membrane, and
- wherein the sensor is configured to be partially inserted into a user's skin.
8 more elements
A method comprising:
- exposing an analyte sensor to a body fluid comprising at least one of glucose and ethanol, the analyte sensor comprising:
- a) a working electrode;
- b) a glucose-responsive active area disposed upon a surface of the working electrode, the glucose-responsive active area comprising a glucose-responsive active enzyme capable of generating a first signal at the working electrode proportional to a glucose concentration;
- c) an ethanol-responsive active area comprising:
- i) a first active area disposed directly upon the surface of the working electrode spaced apart from the glucose-responsive active area, the first active area comprising xanthine oxidase and an electron transfer agent, in amounts sufficient to react with acetaldehyde to generate a second signal at the working electrode proportional to an alcohol concentration;
- and
- ii) a second active area isolated from the working electrode, the second active area comprising glucose oxidase and catalase in amounts sufficient to form acetaldehyde upon exposure to ethanol;
- d) a first membrane permeable to acetaldehyde disposed upon the first active area;
- e) a second membrane permeable to glucose and ethanol disposed upon the glucose-responsive active area and the second active area, and
- detecting a first signal proportional to a glucose concentration and the second signal proportional to an ethanol concentration,
- wherein the second active area is disposed directly upon the first membrane, and
- wherein the sensor is configured to be partially inserted into a user's skin.
9 more elements
Dependencies are inferred from the claim text.