# US 12,217,884 B2 · Protein-based films

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- Number: US 12,217,884 B2
- Document ID: US12217884B2
- Status: Granted
- Kind: B2
- Application number: 17/772,812
- Filed: 2020-10-29
- Published: 2023-01-05
- Granted: 2025-02-04
- Earliest claimed: 2019-10-29
- Assignees: Technion Research & Development Foundation Limited
- Applicants: Technion Research & Development Foundation Limited
- Inventors: Nadav Amdursky; Ramesh Nandi
- Web page: https://patentgrep.com/patent/US12217884B2

## CPC

- C07K 14/765: Serum albumin, e.g. HSA
- H01B 1/122: {Ionic conductors}
- C07K 14/76: Albumins
- H10K 85/761: {Biomolecules or bio-macromolecules, e.g. proteins, chlorophyl, lipids or enzymes}
- Y02E 10/549: Organic PV cells
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- H01B: CABLES; CONDUCTORS; INSULATORS; SELECTION OF MATERIALS FOR THEIR CONDUCTIVE, INSULATING OR DIELECTRIC PROPERTIES (selection for magnetic properties H01F1/00; waveguides H01P {; printed circuits H05K})
- H10K: ORGANIC ELECTRIC SOLID-STATE DEVICES
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

## Abstract

Protein based highly stretchable compositions with ionic conductivity are disclosed herein. Methods for preparing such compositions are also disclosed.

## Claims

### Claim 1

A composition comprising an at least partially denatured protein crosslinked via one or more covalent crosslinkers, wherein said composition is in a form of a film and is characterized by a conductivity in the range of 0.1 mS cm−1 to 1.5 mS cm−1; and wherein said composition further comprises a disulfide bond reducing agent comprising one or more thiol containing reducing agents.

### Claim 2 (depends on 1)

The composition of claim 1, characterized by a transparency in the range of 95% to 100%; and wherein said disulfide bond reducing agent comprises one or more thiol containing reducing agent, including but not limited to 2-mercaptoethanol, dithiothreitol (DTT), (tris(2-carboxyethyl) phosphine) (TCEP), cysteine, glutathione, a phosphine-containing agent or any combination thereof.

### Claim 3 (depends on 1)

The composition of claim 1, characterized by a Young's Modulus in the range of 1 MPa to 50 MPa.

### Claim 4 (depends on 1)

The composition of claim 1, characterized by an elastic limit in the range of 100% to 600%.

### Claim 5 (depends on 1)

The composition of claim 1, wherein said one or more covalent crosslinkers comprise an amine, diamine or dithiol.

### Claim 6 (depends on 1)

The composition of claim 1, wherein said at least partially denatured protein is selected from: (i) a protein crosslinked via amine bonds or disulfide bonds; (ii) a protein characterized by a conductance threshold of at least 0.1 mS cm−1; and (iii) a protein comprising albumin.

### Claim 7 (depends on 1)

The composition of claim 1, wherein said at least partially denatured protein and said one or more covalent crosslinkers are present within said composition in a weight per weight (w/w) ratio of 100:0.01 to 100:1.

### Claim 8 (depends on 1)

The composition of claim 1, comprising 0.01% to 25% (w/w) of said disulfide bond reducing agent.

### Claim 9 (depends on 1)

The composition of claim 1, wherein the at least partially denatured protein is bovine serum albumin (BSA) and wherein said one or more covalent crosslinkers is dithiolethylenediamine.

### Claim 10 (depends on 1)

The composition of claim 1, characterized by a fluorescence emission in the range of 350 nm to 750 nm.

### Claim 11 (depends on 1)

The composition of claim 1, further comprising a dopant, a light activated molecule, or both; optionally wherein said light activated molecule is selected from a photobase generator or a photoacid generator; optionally wherein said dopant is selected from a polymer, an organic molecule, an inorganic molecule, an inorganic nanocrystal, a nanowire, and any combination thereof.

### Claim 12 (depends on 1)

The composition of claim 1, further comprising a chemical modification of said at least partially denatured protein selected from fluorination, sulfonation, phosphorylation, carboxylation, amide bond formation, glycosylation, or any combination thereof.

### Claim 13 (depends on 1)

The composition of claim 1, characterized by a conductivity in the range of 1.5 mS cm-1 to 100 mS cm -1.