# US 12,216,076 B2 · Vascular sap measurement sensor > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,216,076 B2 - Document ID: US12216076B2 - Status: Granted - Kind: B2 - Application number: 17/767,897 - Filed: 2020-10-08 - Published: 2024-03-14 - Granted: 2025-02-04 - Earliest claimed: 2019-10-10 - Assignees: National University Corporation Kagawa University - Applicants: National University Corporation Kagawa University - Inventors: Fusao Shimokawa; Kazuma Ishida - Web page: https://patentgrep.com/patent/US12216076B2 ## CPC - G01N 27/414: Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS - A01G 7/00: Botany in general - G01N 27/07: Construction of measuring vessels; Electrodes therefor - G01N 27/416: Systems (G01N27/27 takes precedence) - G01N 27/06: of a liquid (involving electrolysis G01N27/26) - G01N 33/0098: {Plants or trees (wood G01N33/46)} - G01N 2333/415: from plants - G01R 1/06744: {Microprobes, i.e. having dimensions as IC details} - G01R 1/06783: {containing liquids} - G01R 1/0735: {arranged on a flexible frame or film} - G01R 1/07357: {with flexible bodies, e.g. buckling beams} - G01R 1/07371: {using an intermediate card or back card with apertures through which the probes pass} - G01R 27/22: Measuring resistance of fluids - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - A01G: HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING (picking of fruits, vegetables, hops or the like A01D46/00; propagating unicellular algae C12N1/12) - G01R: MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES (indicating correct tuning of resonant circuits H03J3/12) ## Abstract A vascular sap measurement sensor includes an indicator electrode probe, a reference electrode probe, and a supporting portion. The indicator electrode probe is an ion-sensitive field effect transistor. The reference electrode probe includes a solid reference electrode, the solid reference electrode including a base layer, a silver chloride layer, and a chloride layer, the base layer being formed of an electrically conductive body, the silver chloride layer being formed on a surface of the base layer, the chloride layer being formed on a surface of the silver chloride layer. The supporting portion supports the indicator electrode probe and the reference electrode probe arranged in parallel. ## Claims ### Claim 1 A vascular sap measurement sensor comprising: an indicator electrode probe that includes an indicator electrode which is an ion-sensitive field effect transistor; a reference electrode probe that includes a solid reference electrode, the solid reference electrode including a base layer, a silver chloride layer, and a chloride layer, the base layer being formed of an electrically conductive body, the silver chloride layer being formed on a surface of the base layer, the chloride layer being formed on a surface of the silver chloride layer; an electrical conductivity probe that includes an electrical conductivity electrode pair, the electrical conductivity electrode pair including a pair of electrodes disposed at a predetermined interval; and a supporting portion that supports the indicator electrode probe, the reference electrode probe and the electrical conductivity probe arranged in parallel, wherein each of the pair of electrodes includes a metal layer that covers a top surface and side surfaces of a projection formed on a probe surface. ### Claim 2 (depends on 1) The vascular sap measurement sensor according to claim 1, comprising a temperature probe that includes a temperature sensor, wherein the temperature probe is supported by the supporting portion. ### Claim 3 (depends on 1) The vascular sap measurement sensor according to claim 1, wherein the electrical conductivity electrode pair has a cell constant of 500 to 2,000 m−1. ### Claim 4 (depends on 1) The vascular sap measurement sensor according to claim 1, wherein the pair of electrodes are arranged side by side along a width direction of the electrical conductivity probe. ### Claim 5 (depends on 1) The vascular sap measurement sensor according to claim 1, wherein the pair of electrodes are arranged side by side along an axial direction of the electrical conductivity probe. ### Claim 6 (depends on 1) The vascular sap measurement sensor according to claim 1, wherein the indicator electrode, the solid reference electrode, and the electrical conductivity electrode pair are disposed at a same position in a sticking direction to a plant. ### Claim 7 (depends on 1) The vascular sap measurement sensor according to claim 1, wherein the indicator electrode and the solid reference electrode are disposed at positions different from the electrical conductivity electrode pair in a sticking direction to a plant, and the electrical conductivity electrode pair is disposed in a xylem of the plant with the indicator electrode and the solid reference electrode disposed in a phloem of the plant. ### Claim 8 (depends on 1) The vascular sap measurement sensor according to claim 1, comprising: a heater-equipped temperature probe that includes a temperature sensor and a heater; and a temperature probe that includes a temperature sensor, wherein the heater-equipped temperature probe and the temperature probe are supported by the supporting portion. ### Claim 9 (depends on 8) The vascular sap measurement sensor according to claim 8, further comprising two of the temperature probes, wherein the two temperature probes are disposed at positions across the heater-equipped temperature probe. ### Claim 10 A vascular sap measurement sensor, comprising: an indicator electrode probe that includes an indicator electrode which is an ion-sensitive field effect transistor; a reference electrode probe that includes a solid reference electrode, the solid reference electrode including a base layer, a silver chloride layer, and a chloride layer, the base layer being formed of an electrically conductive body, the silver chloride layer being formed on a surface of the base layer, the chloride layer being formed on a surface of the silver chloride layer; and a supporting portion that supports the indicator electrode probe and the reference electrode probe arranged in parallel, wherein the chloride layer is produced by mixing and solidifying a glass paste and a potassium chloride at a weight ratio of 1:0.05 to 0.10.