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US 12,216,032 B2Granted
Device and method for the dissociation of tissue
AssigneeFraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
InventorsChristian Reis, Stefan Scheuermann, Armin Schäfer, Daniel Edinger
Life of this patent
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Abstract
The invention relates to a device and a method for the dissociation of tissue. In one embodiment, the device has at least one dissociation unit, for receiving a tissue sample, which is disposed at least partially in a pot-shaped cell strainer. In addition, the invention relates, in one embodiment, to a device and a method for the dissociation of tissue, the tissue being dissociated by a grinder having mutually movable rows of teeth which cut the tissue when moving past each other in the one direction and grind the tissue when moving past each other in the opposite direction.
Claims28
A device for dissociation of tissue, the device comprising:
- at least one dissociation unit for receiving a tissue sample;
- and
- at least one cell strainer, wherein the at least one dissociation unit is disposed at least partially in the at least one cell strainer, and wherein the at least one dissociation unit includes:
- at least one vessel for receiving the tissue sample;
- and
- a grinder disposed in the at least one vessel, wherein the grinder includes at least one first row of teeth with a plurality of teeth and at least one second row of teeth with a second plurality of teeth, the at least one first row of teeth being disposed on a base of the at least one vessel and the at least one second row of teeth being disposed on a cover of the at least one vessel, the at least one first row of teeth being movable relative to the at least one second row of teeth so that the teeth of the at least one first row of teeth move past the teeth of the at least one second row of teeth, wherein one of the at least one first row of teeth and one of the at least one second row of teeth are disposed at such a spacing respectively adjacent to each other so that the teeth of the respectively adjacent rows of teeth, when they are moved past with respect to each other, effect a dissociation of a part of the tissue sample in a spacing region by their minimum spacing relative to each other.
3 more elements
A device for dissociation of tissue, comprising:
- at least one vessel for receiving a tissue sample;
- and
- a grinder disposed in the at least one vessel, wherein the grinder includes at least two mutually movable rows of teeth, wherein each of the at least two mutually movable rows of teeth include a plurality of teeth, wherein adjacent ones of the at least two mutually movable rows of teeth being disposed at such a spacing relative to each other that the teeth of these respectively adjacent rows of teeth effect a dissociation of a part of the tissue sample in a spacing region by their minimum spacing relative to each other, wherein the respectively adjacent rows of teeth are configured such that, when they move past each other in a first direction, the respectively adjacent rows of teeth cut at least part of the tissue sample and, when the respectively adjacent rows of teeth move past each other in a second direction opposite to the first direction, grind at least part of the tissue sample, wherein the respectively adjacent rows of teeth extend in a common plane and are mutually movable in the common plane, wherein the teeth of the respectively adjacent rows of teeth have a cross-section parallel to the common plane, which has a pointed corner and a rounded or blunt side situated opposite the pointed corner, and wherein the pointed corners of teeth of the respectively adjacent rows of teeth are directed in opposite directions.
A method for dissociation of tissue, the method comprising:
- introducing a tissue sample being into a device, the device comprising:
- at least one dissociation unit for receiving the tissue sample;
- and
- at least one cell strainer, wherein the at least one dissociation unit is disposed at least partially in the at least one cell strainer;
- at least one vessel for receiving the tissue sample;
- and
- at least one grinder disposed in the at least one vessel, wherein the at least one grinder includes a first row of teeth, the first row of teeth including a first plurality of teeth, and a second row of teeth, the second row of teeth including a second plurality of teeth, wherein the first row of teeth is located on a base of the at least one vessel and the second row of teeth are located on a cover of the at least one vessel, wherein the first row of teeth is movable relative to the second row of teeth so that the teeth of the first row of teeth move past the teeth of the second row of teeth, wherein the first row of teeth and the second row of teeth are spaced respectively adjacent to each other so that the teeth of the first row of teeth and the teeth of the second row of teeth, when they are moved past each other, effect a dissociation of a part of the tissue sample in a spacing region representing a minimum spacing of the first row of teeth and the second row of teeth relative to each other;
- and
- actuating at least one of the dissociation unit or the grinder.
6 more elements
Dependencies are inferred from the claim text.