# US 12,215,385 B2 · System and method for providing biology-based anti-tamper > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,215,385 B2 - Document ID: US12215385B2 - Status: Granted - Kind: B2 - Application number: 16/120,987 - Filed: 2018-09-04 - Published: 2020-03-05 - Granted: 2025-02-04 - Earliest claimed: 2018-09-04 - Assignees: Northrop Grumman Systems Corporation - Applicants: Northrop Grumman Systems Corporation - Inventors: David A. Selck; Om Prakash; Lito Medalle - Web page: https://patentgrep.com/patent/US12215385B2 ## CPC - C12Q 1/6876: Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes - C12Q 1/04: Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} - C12Q 1/6816: characterised by the detection means (C12Q1/6804 takes precedence) - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES ## Abstract A system for biology based anti-tamper and a method for detecting a tampering activity to an object using biological materials are provided. The system includes a biological medium that includes a biological member such as deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and/or live or hibernating organism. The biological medium is applied to a portion of an object that is required to monitor whether any tampering may have occurred. The biological member is engineered to change its state when exposed to a stimulus. Upon suspicion, the state of the biological member is examined to find if there is any change of state. The biological member is capable of being barcoded, which provides an extra security measure. ## Claims ### Claim 1 An anti-tamper system installed at a portion of an object and configured to detect a tampering activity to the object, comprising: a biological member including at least one biological material that is engineered to change a state when exposed to one or more stimuli and is engineered such that the change of the state is detectable, wherein the biological material includes a ribonucleic acid (RNA) strand including at least three fixed domains and a plurality of variable domains, wherein among the at least three fixed domains two fixed domains are located closer to each other within the RNA strand relative to the third fixed domain, thereby rendering sections of nucleic acids of varying size located between the fixed domains, wherein exposure of the RNA to one or more stimuli can degrade one or more sections of nucleic acids located between the fixed domains into one or more strands of varying size thereby showing the extent of degradation of the RNA; and a medium disposed at the portion of the object to form a coating of the biological member, wherein the medium contains the biological member and holds the biological member in contact with the object, and wherein the medium comprises water and includes trehalose as a stabilization agent to ensure stability of the biological member in the medium. ### Claim 2 (depends on 1) The anti-tamper system of claim 1 wherein the at least one biological material includes a deoxyribonucleic acid (DNA), or a live or hibernating organism. ### Claim 3 (depends on 1) The anti-tamper system of claim 1 wherein the at least one biological material includes a DNA fragment and the medium is a form of a coating that is configured to cover a portion of the object that will be affected if the object is disassembled. ### Claim 4 (depends on 3) The anti-tamper system of claim 3 wherein the DNA fragment is engineered to have a barcode. ### Claim 5 (depends on 1) The anti-tamper system of claim 1 wherein the portion of the object onto which the medium containing the RNA strand is disposed of co is not exposed to the one or more stimuli unless tampering activity of the object occurs. ### Claim 6 (depends on 1) The anti-tamper system of claim 1 wherein the sections of nucleic acids are located within the variable domains of the RNA strand, and the variable domains are barcoded. ### Claim 7 (depends on 1) The anti-tamper system of claim 1 wherein the at least one biological material includes live or hibernating organisms and the medium is configured to be disposed in a portion of the object in which the live or hibernating organism is not exposed to the one or more stimuli unless tampering activity of the object occurs. ### Claim 8 (depends on 7) The anti-tamper system of claim 7 wherein the live or hibernating organism is engineered to have a barcode. ### Claim 9 An anti-tamper system installed at a portion of an object and configured to detect a tampering activity to the object, comprising: one or more biological anti-tamper mediums comprising: a biological member formed on the object; and a medium that is disposed at the portion of the object to form a coating of the biological member, contains the biological member and holds the biological member in contact with the object, wherein: the biological member includes at least one biological material that is engineered to change a state when exposed to one or more stimuli and is engineered such that the change of the state is detectable, wherein the biological material includes a ribonucleic acid (RNA) strand including at least three fixed domains and a plurality of variable domains, wherein among the at least three fixed domains two fixed domains are located closer to each other within the RNA strand relative to the third fixed domain, thereby rendering sections of nucleic acids of varying size located between the fixed domains, wherein exposure of the RNA to one or more stimuli can degrade one or more sections of nucleic acids located between the fixed domains into one or more strands of varying size thereby showing the extent of degradation of the RNA; and the plurality of the variable domains include barcoded variable domains, and the barcoded variable domains are barcoded differently from each other. ### Claim 10 (depends on 9) The anti-tamper system of claim 9 wherein the at least one biological material of the biological anti-tamper mediums further includes: a DNA fragment suspended in one of the biological anti-tamper mediums that is configured to be formed on the object as a coating that is configured to cover a portion of the object that will be affected if the object is disassembled; or live or hibernating organisms suspended in another of the biological anti-tamper mediums that is configured to be disposed in a portion of the object in which the live or hibernating organism is not exposed to one or more of the one or more stimuli unless tampering activity of that portion of the object occurs. ### Claim 11 (depends on 1) The anti-tamper system of claim 1 wherein the at least one biological material is engineered such that the change of the state is detectable by polymerase chain reaction (RTPCR). ### Claim 12 (depends on 9) The anti-tamper system of claim 9 wherein the at least one biological material is engineered such that the change of the state is detectable by polymerase chain reaction (RTPCR). ### Claim 13 (depends on 1) The anti-tamper system of claim 1 wherein the medium comprises Tris (Hydroxymethyl) aminomethane (TRIS). ### Claim 14 (depends on 13) The anti-tamper system of claim 13 wherein degradation of the nucleic acid is tracked using reverse transcription followed by polymerase chain reaction (RTPCR).