# US 12,215,360 B2 · Encapsulation of dual-enzyme composition for preventing, treating and/or alleviating veisalgia and symptoms associated therewith

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 12,215,360 B2
- Document ID: US12215360B2
- Status: Granted
- Kind: B2
- Application number: 18/465,961
- Filed: 2023-09-12
- Published: 2024-07-04
- Granted: 2025-02-04
- Earliest claimed: 2021-05-05
- Assignees: Bing Biotech Limited
- Applicants: Bing Biotech Limited
- Inventors: Bing Lou Wong; Sek Lun Law; Joo Ann Ewe; Chun Hay Ko
- Web page: https://patentgrep.com/patent/US12215360B2

## CPC

- C12N 9/0006: {acting on CH-OH groups as donors (1.1)}
- C12N 9/0008: {acting on the aldehyde or oxo group of donors (1.2)}
- C12Y 101/01001: Alcohol dehydrogenase (1.1.1.1)
- C12Y 102/01003: Aldehyde dehydrogenase (NAD+) (1.2.1.3)
- A61K 38/44: Oxidoreductases (1)
- Y02E 50/10: Biofuels, e.g. bio-diesel
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

## Abstract

An encapsulated composition for converting ethanol to acetaldehyde and subsequently converting the acetaldehyde to acetate. The composition includes alcohol dehydrogenase and aldehyde dehydrogenase in a molar ratio of approximately 1:3 to approximately 1:51. The composition is encapsulated by a polysaccharide-whey-casein encapsulant such that the encapsulated composition has a controlled release property in the small intestine. The composition reduces symptoms caused by over-consumption of alcohol.

## Claims

### Claim 1

An encapsulated composition for converting ethanol to acetaldehyde and subsequently converting the acetaldehyde to acetate, the composition comprising alcohol dehydrogenase and aldehyde dehydrogenase in a molar ratio of approximately 1:3 to approximately 1:51, the composition being encapsulated by a polysaccharide-whey-casein encapsulant such that the encapsulated composition is an orally-administered controlled release composition in the small intestine.

### Claim 2 (depends on 1)

The encapsulated composition of claim 1, wherein the alcohol dehydrogenase and aldehyde dehydrogenase are sourced from the same or two or more different animal origins.

### Claim 3 (depends on 2)

The encapsulated composition of claim 2, wherein the alcohol dehydrogenase and aldehyde dehydrogenase are sourced from the same animal origin, and the animal is selected from bovine, ovine, equine, or galline.

### Claim 4 (depends on 3)

The encapsulated composition of claim 3, wherein the alcohol dehydrogenase and aldehyde dehydrogenase are sourced from different animal origins, and the animals is selected from two or more of bovine, ovine, equine, galline or any combination thereof.

### Claim 5 (depends on 3)

The encapsulated composition of claim 3, wherein the alcohol dehydrogenase and aldehyde dehydrogenase are sourced from the livers of the animals.

### Claim 6 (depends on 1)

The encapsulated composition of claim 1, wherein the alcohol dehydrogenase and aldehyde dehydrogenase are sourced from Baker's yeast (S. cerevisiae).

### Claim 7 (depends on 1)

The encapsulated composition of claim 1, wherein the encapsulated composition includes 0.6% w/v alginate or less, 5% w/v whey protein or less and 50% w/v milk or less, with a balance being alcohol dehydrogenase and aldehyde dehydrogenase in a molar ratio of approximately 1:3 to approximately 1:51.

### Claim 8 (depends on 1)

The encapsulated composition of claim 1, wherein a particle size of the encapsulated composition is in a range of 0.2-2 mm.

### Claim 9 (depends on 1)

The encapsulated composition of claim 1, wherein the polysaccharide is one or more of alginate, chitosan, pectin, starch, cellulose, agarose, xanthan gum, carrageenan, or guar gum.

### Claim 10 (depends on 1)

The encapsulated composition of claim 1, wherein the polysaccharide is sodium alginate.

### Claim 11 (depends on 1)

A method of preparing the encapsulated composition of claim 1, comprising:
forming a mixture of polysaccharide, whey, and milk;
introducing the alcohol dehydrogenase and aldehyde dehydrogenase composition in a molar ratio of approximately 1:3 to approximately 1:51 to the mixture;
forming particles of the alcohol dehydrogenase and aldehyde dehydrogenase composition in a molar ratio of approximately 1:3 to approximately 1:51 surrounded by the encapsulant formed from the polysaccharide, whey, and milk mixture.

### Claim 12 (depends on 11)

The method of claim 11, wherein the polysaccharide is sodium alginate.