Xylose metabolizing yeast
AssigneeBASF SE
InventorsHaowen Xu, Dominik Satory, Christopher Jackson
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Abstract
Described herein are microorganisms, in particular yeast, which have been transformed with one or more expression construct(s) for i) the overexpression of the native genes encoding xylulose kinase (XKS1), transaldolase (TAL1), transketolase 1 (TKL1) and transketolase 2 (TKL2) and ii) the expression of a functional heterologous gene encoding a xylose isomerase (XI), where the xylose isomerase (XI) gene is derived from a microorganism selected from the group consisting of T. neapolitana, A. andensis and C. clariflavum. Also described herein are expression constructs, methods for fermenting pentose sugars using the microorganisms and methods for producing such microorganisms.
Claims7
A method for fermenting pentose sugar comprising culturing a microorganism in a culture medium comprising pentose sugar(s) under conditions in which the pentose sugar(s) can be metabolized, wherein the microorganism has been transformed with a single expression construct(s) for
- i) the overexpression of native genes encoding xylulose kinase (XKS1), transaldolase (TAL1), transketolase 1 (TKL1) and transketolase 2 (TKL2) and
- ii) the expression of a functional heterologous gene encoding a xylose isomerase (XI), wherein the xylose isomerase (XI) gene is encoded by a nucleic acid sequence having at least 95% sequence identify to SEQ ID No 21,
- wherein the xylulose kinase (XKS1) is encoded by a nucleic acid sequence having at least 95% sequence identity to SEQ ID No 74, the transaldolase (TAL1) is encoded by a nucleic acid sequence having at least 95% sequence identity to SEQ ID No 77, the transketolase 1 (TKL1) is encoded by a nucleic acid sequence having at least 95% sequence identity to SEQ ID No 80 and the transketolase 2 (TKL2) is encoded by a nucleic acid sequence having at least 95% sequence identity to SEQ ID No 83,
- wherein the xylose isomerase (XI) gene is under the control of a constitutive promoter of Saccharomyces cerevisiae;
- wherein the pentose sugar is xylose;
- and
- wherein the microorganism is a yeast of the species Saccharomyces cerevisiae.
A method of using a microorganism, the method comprising fermenting pentose sugar(s) using the microorganism, wherein the microorganism has been transformed with a single expression construct(s) for
- i) the overexpression of the native genes for xylulose kinase (XKS1), transaldolase (TAL1), transketolase 1 (TKL1) and transketolase 2 (TKL2), and
- ii) the expression of a functional heterologous gene encoding a xylose isomerase (XI), wherein the xylose isomerase (XI) is encoded by a nucleic acid sequence having at least 95% sequence identity to SEQ ID No 21,
- wherein the xylulose kinase (XKS1) is encoded by a nucleic acid sequence having at least 95% sequence identity to SEQ ID No 74, the transaldolase (TAL1) is encoded by nucleic acid sequence having at least 95% sequence identity to SEQ ID No 77, the transketolase 1 (TKL1) is encoded by a nucleic acid sequence having at least 95% sequence identity to SEQ ID No 80 and the transketolase 2 (TKL2) is encoded by a nucleic acid sequence having at least 95% sequence identity to SEQ ID No 83,
- wherein the xylose isomerase (XI) gene is under the control of a constitutive promoter of Saccharomyces cerevisiae;
- wherein the pentose sugar is xylose;
- and
- wherein the microorganism is a yeast of the species Saccharomyces cerevisiae.
Dependencies are inferred from the claim text.