# US 12,215,263 B2 · Tracer composition > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,215,263 B2 - Document ID: US12215263B2 - Status: Granted - Kind: B2 - Application number: 17/599,944 - Filed: 2020-03-26 - Published: 2022-06-23 - Granted: 2025-02-04 - Earliest claimed: 2019-03-29 - Assignees: Chelsea Technologies Ltd - Applicants: Chelsea Technologies Ltd - Inventors: Jerry Cross; Charles Whitfield; John Attridge - Web page: https://patentgrep.com/patent/US12215263B2 ## CPC - C09K 11/06: containing organic luminescent materials - A01N 25/00: Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application {, e.g. seed treatment or sequential application}; Substances for reducing the noxious effect of the active ingredients to organisms other than pests - A01N 25/04: Dispersions, {emulsions, suspoemulsions, suspension concentrates} or gels (foams A01N25/16) - C09K 11/025: {non-luminescent particle coatings or suspension media} - G01N 21/6428: {Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53)} - C09K 2211/1018: Heterocyclic compounds - C09K: MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract The disclosure provides a tracer composition. The composition comprises a fluorescent molecule which fluoresces when exposed to an excitation wavelength, and thereby emit a photon with a wavelength between 400 and 600 nm; and a gelling agent. ## Claims ### Claim 1 A food safe tracer composition, the composition comprising: (i) a food safe fluorescent molecule which fluoresces when exposed to an excitation wavelength, and thereby emits a photon with a wavelength between 400 and 600 nm, wherein the food safe fluorescent molecule is a compound of formula (I): wherein each of R1 to R8 is independently hydrogen or a C1-5 alkyl, or a salt and/or solvate thereof; (ii) a stabilizing agent configured to improve the photostability of the fluorescent molecule, wherein the stabilizing agent comprises thiosulfuric acid or a salt and/or solvate thereof or a compound of formula (II): wherein each of R9 to R12 is independently hydrogen or a C1-5 alkyl or a salt and/or solvate thereof; and (iii) a gelling agent, wherein the gelling agent comprises a saccharide and an alcohol, and the weight ratio of the saccharide to the alcohol is between 1:10 and 10:1. ### Claim 2 (depends on 1) The tracer composition according to claim 1, wherein the fluorescent molecule emits a photon with a wavelength between 500 and 600 nm. ### Claim 3 (depends on 1) The tracer composition according to claim 1, wherein the fluorescent molecule fluoresces when exposed to an excitation wavelength between 150 and 700nm, optionally wherein the fluorescent molecule fluoresces when exposed to an excitation wavelength between 465 and 500 nm. ### Claim 4 (depends on 1) The tracer composition according to claim 1, wherein the fluorescent molecule riboflavin 5′-monophosphate or a salt and/or solvate thereof. ### Claim 5 (depends on 1) The tracer composition according to claim 1, wherein the tracer composition comprises: the florescent molecule at a concentration between 0.0001 and 50 wt %, between 0.001and 50 wt %, between 0.005 and 10 wt %, between 0.01 and 1 wt %, between 0.02 and 0.8 wt %, between 0.04 and 0.6 wt %, between 0.06 and 0.4 wt % or between 0.08 and 0.2 wt %; and/or the gelling agent at a concentration of between 10 and 99 wt %, between 20 and 95 wt %, between 30 and 90 wt %, between 40 and 85 wt %, between 50 and 80 wt %, between 60 and 77.5wt %, between 65 and 75 wt % or between 67.5 and 72.5 wt %. ### Claim 6 (depends on 1) The tracer composition according to claim 1, wherein the saccharide is a disaccharide, or a salt and/or solvate thereof, optionally wherein the disaccharide is sucrose, or a salt and/or solvate thereof. ### Claim 7 (depends on 1) The tracer composition according to claim 1, wherein the weight ratio of the saccharide to the alcohol is between 5:1 and 1:5, between 1:4 and 4:1, between 1:3 and 3:1, between 1:2 and 2:1, between 1.75:1 and 1:1, between 1.5:1 and 1.1:1 or between 1.3:1 and 1.2:1. ### Claim 8 (depends on 1) The tracer composition according to claim 1, wherein the stabilizing agent absorbs photons with a wavelength between 100 and 500 nm, between 150 and 400 nm, between 175 and 350 nm or between 200 and 325 nm, optionally wherein the tracer composition comprises the stabilizing agent at a concentration of between 0.01 and 90 wt %, between 0.1 and 50 wt %, between 0.5 and 30 wt %, between 1 and 10 wt %, between 2 and 7.5 wt %, between 2.5 and 5 wt %, between 3 and 4.5 wt % or between 3.5 and 4 wt %. ### Claim 9 (depends on 1) The tracer composition according to claim 1, wherein the stabilizing agent comprises a compound of formula (II): wherein each of R9 to R12 is independently hydrogen or a C1-5 alkyl, or a salt and/or solvate thereof, optionally wherein the stabilizing agent comprises ascorbic acid, or a salt and/or solvate thereof. ### Claim 10 (depends on 1) The tracer composition according to claim 1, wherein the tracer composition comprises a solvent, which preferably comprises water. ### Claim 11 (depends on 1) A plant protection composition comprising: the tracer composition of claim 1; and a plant protection product (PPP). ### Claim 12 (depends on 11) The plant protection composition according to claim 11, wherein the PPP comprises a herbicide, an insecticide, an acaricide, a nematicide, a molluscicide, a bactericide and/or a fungicide, and/or wherein the plant protection composition comprises the tracer composition in an amount which is between 0.01 and 50% (v/v), between 0.1 and 40% (v/v), between 0.25 and 30% (v/v), between 0.5 and 20% (v/v), between 0.75 and 10% (v/v) or between 1 and 5% (v/v), and/or wherein the plant protection composition comprises the PPP in an amount which is between 20 and 99.99% (v/v), between 60 and 99.9% (v/v), between 40 and 99.9% (v/v), between 90 and 99% (v/v) or between 95 and 99% (v/v). ### Claim 13 An apparatus comprising: a dual wavelength light emitting device to selectively illuminate a surface at a first wavelength and to selectively illuminate the surface at a second wavelength, wherein the surface has a material thereon and wherein the first wavelength is in the range of 400 to 500 nm and the second wavelength is in the range of 500 to 600 nm; a camera sensor for generating a first fluorescence image in response to the illumination of said surface at the first wavelength and a first visual image in response to the illumination of said surface at the second wavelength; and a processor configured to process the first visual image to generate a mask indicative of an area of the first visual image; generate a second fluorescence image by masking the first fluorescence image using the mask; and process the second fluorescence image to determine a coverage information and/or a dosage information of said material. ### Claim 14 (depends on 13) An apparatus according to claim 13, wherein the light emitting device comprises at least one light emitting diode, optionally wherein each of said at least one light emitting diodes is placed in a back focal plane of said light emitting device to provide a uniform pattern of light to illuminate said surface. ### Claim 15 (depends on 13) An apparatus according to claim 13, wherein the light emitting device further comprises at least one of: a first planoconvex lens; at least one emission filter; and a second planoconvex; and/or wherein the surface comprises a plant or a part thereof. ### Claim 16 A method comprising: selectively illuminating a surface at a first wavelength, wherein the surface has a material thereon and the first wavelength is in the range of 400 to 500 nm; generating a first fluorescence image of said surface in response to the illumination at the first wavelength; selectively illuminating the surface at a second wavelength, wherein the second wavelength is in the range of 500 to 600 nm; generating a first visual image of said surface in response to the illumination at the second wavelength; processing the first visual image to generate a mask indicative of an area of the first visual image; generating a second fluorescence image by masking the first fluorescence image using the mask; and processing the second fluorescence image to determine a coverage information and/or a dosage information of said material. ### Claim 17 (depends on 1, 16) A method according to claim 16, wherein the material is the tracer composition defined by claim 1. ### Claim 18 (depends on 16) A method according to claim 16, wherein the method further comprises: generating a plurality of candidate first fluorescence images at different illumination intensities; and selecting one of said plurality candidate first fluorescence images as said first fluorescence image.