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US 12,215,078 B2Granted
Compositions and methods for protein labeling, modification, analysis, and targeted delivery
AssigneeUniversity of Massachusetts
InventorsSankaran Thayumanavan, Richard W. Vachet, Jiaming Zhuang, Bo Zhao
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Abstract
The invention relates to chemically reactive and/or biologically active compounds, reagents and compositions thereof. More particularly, the invention provides novel reagents that are useful in chemical synthesis, functionalization, delivery, probing and/or analytical measurements of small molecule drugs, proteins, antibodies and other biomolecules. The invention provides novel biologically active agents useful as diagnostics or therapeutics, and related composition and methods of uses thereof.
Claims8
A method for labeling a protein, comprising:
- providing a protein of formula (IV) comprising a nucleophilic group (Nu) at a point of desired labeling:
- R-Nu, (IV)
- wherein R-Nu is R—SH or R—NH2;
- reacting the protein of formula (IV) with a compound of formula (V) having both a label and a reporter group:
- wherein
- R3 is H;
- R4 is H;
- Y is O;
- L is a linking group comprising —(CH2CH2O)n—, wherein n is an integer from 1 to 10, and the reaction results in releasing of the reporter and formation of a labeled protein of formula (VI)
- and
- detecting a signal of the reporter and/or the presence of the label to analyze or quantify the protein.
8 more elements
Dependencies are inferred from the claim text.