# US 12,214,352 B1 · Microfluidic chip holders and methods of removing chips therefrom

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- Number: US 12,214,352 B1
- Document ID: US12214352B1
- Status: Granted
- Kind: B1
- Application number: 17/685,694
- Filed: 2022-03-03
- Granted: 2025-02-04
- Earliest claimed: 2021-03-03
- Assignees: 10x Genomics, Inc.
- Applicants: 10x Genomics, Inc.
- Inventors: Jennifer Lew; Joaquin Trosper-Torres; Liza S. Man; Alexander Post Kindwall; Kevin Gilmartin; David Morgan
- Web page: https://patentgrep.com/patent/US12214352B1

## CPC

- B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces}
- B01L 9/527: {for microfluidic devices, e.g. used for lab-on-a-chip}
- B01L 2200/025: Align devices or objects to ensure defined positions relative to each other
- B01L 2200/027: for microfluidic devices
- B01L 2200/04: Exchange or ejection of cartridges, containers or reservoirs
- B01L 2300/046: Function or devices integrated in the closure
- B01L 2300/0609: Holders integrated in container to position an object
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

## Abstract

The invention provides microfluidic chip holders, systems containing them, and methods of microfluidic chip removal therefrom that allow easy, reproducible release and removal of microfluidic chips from the holders. Holders of the invention include latching mechanisms that hold a chip securely in position when locked and eject the chip when unlocked, allowing easier grasping of the chip and reducing the likelihood that the fluidic contents of the chip are disturbed during removal.

## Claims

### Claim 1

A method of separating a microfluidic chip from a microfluidic chip holder, the method comprising:
a) providing the microfluidic chip holder comprising i) a receptacle with a base that holds the microfluidic chip in a planar conformation and ii) a latching mechanism comprising a latch that holds the chip in the receptacle and is mechanically coupled to an effort side of a lever with a resistance side of the lever under an edge or a catching feature on the edge of the chip;
b) pressing laterally or annularly on the latch to withdraw the latch and apply downward force on the effort side of the lever, thereby releasing and raising the microfluidic chip; and
c) lifting the raised microfluidic chip out of the microfluidic chip holder.

### Claim 2 (depends on 1)

The method of claim 1, wherein step (b) comprises raising the edge of the microfluidic chip in contact with the lever such that the microfluidic chip and the base of the chip holder make an angle that is less than 2° or raising the edge of the microfluidic chip about 0.5 to 2 mm from the base.

### Claim 3 (depends on 1)

The method of claim 1, wherein the latching mechanism comprises a spring and the pressing of step (b) applies stress to the spring to withdraw the latch; or wherein the latching mechanism comprises a fulcrum between the effort and resistance sides of the lever and wherein step (b) comprises causing the lever to pivot on the fulcrum when pressing laterally or annularly on the latch.

### Claim 4 (depends on 1)

The method of claim 1, wherein step (b) comprises annularly or laterally moving a projection on the latch that is disposed at least partially within a groove in the lever against the effort side of the lever, thereby raising the resistance side of the lever and the microfluidic chip.

### Claim 5

A microfluidic chip holder comprising:
a) a receptacle with a base configured to hold a microfluidic chip in a substantially planar conformation; and
b) a latching mechanism comprising a latch and a lever having an effort side and a resistance side, wherein the latch is mechanically coupled to the effort side of the lever and the resistance side of the lever is disposed beneath an edge or a catching feature on the edge of the microfluidic chip when in the receptacle, wherein lateral or annular movement of the latch applies downward force on the effort side of the lever which raises the resistance side of the lever and ejects the microfluidic chip from the receptacle.

### Claim 6 (depends on 5)

The holder of claim 5, wherein the lever is configured to raise the edge of the microfluidic chip to make an angle that is less than 2° with the base; or wherein the lever is configured to raise the edge of the microfluidic chip by about 0.5 to 2 mm.

### Claim 7 (depends on 5)

The holder of claim 5, wherein the lever is configured to raise the edge of the microfluidic chip by about 1 mm.

### Claim 8 (depends on 5)

The holder of claim 5, wherein the latching mechanism further comprises a spring or a fulcrum; or wherein the lever comprises a groove and the latch comprises a projection disposed at least partially within the groove; or wherein the lever is a compound lever.

### Claim 9 (depends on 5)

The holder of claim 5, further comprising a cover that at least partially covers the microfluidic chip when in the receptacle.

### Claim 10 (depends on 9)

The holder of claim 9, wherein the cover is rotatable behind the holder to act as a stand.