Systems and method for detection of analytes in high volumetric flow applications
AssigneeUniversity of Houston System
InventorsRichard C. Willson, Ujwal Patil, Binh V. Vu, Ekaterini Kourentzi, Mary Crum
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Abstract
The present disclosure relates to the detection of analytes in high volumetric flow applications. Particular embodiments relate to the use of fluorescence polarization/anisotropy based for detection of analytes in a flow cell. In one testing format, an analyte of interest is probed with reagents containing fluorescent labeled recognition elements. When present in a sample or portion of a sample, the labeled analyte produces a shift in fluorescence polarization/anisotropy/intensity/lifetime as the output signal following the binding of the recognition elements to the analytes.
Claims32
A method of detecting the presence of a target analyte comprising
- (a) contacting a sample or a separated portion of said sample with a reporter reagent that produces an optically or electromagnetically detectable signal in the presence of the target analyte;
- and
- (b) determining the presence of said target analyte in said sample or said separated portion of said sample by measuring the optically or electromagnetically detectable signal, thereby detecting the presence of said target analyte, wherein
- (i) the sample is a first liquid stream is derived from a primary flow stream resulting from a separative process, or
- (ii) wherein the sample is formed from a separative process selected from the group consisting of chromatography, filtration/ultrafiltration, and precipitation/ZnCl2 precipitation.
A method of chromatographic purification of a target protein comprising:
- (a) introducing a sample comprising a target protein into a chromatographic column;
- (b) capturing the target protein on a chromatographic column matrix in said chromatographic column;
- (c) eluting matrix-captured target protein by change of pH, change of salt concentration, or change of polarity or hydrophobicity of the liquid flowing through the column;
- (d) introducing a reporter reagent into a portion of the liquid leaving the chromatographic column, wherein the reporter agent binds the target protein;
- and
- (e) measuring the concentration of the target protein in the liquid leaving the chromatographic column by detecting the reporter agent.
Dependencies are inferred from the claim text.