# US 12,214,191 B2 · Compositions and methods of applying alternating electric fields to pluripotent stem cells

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- Number: US 12,214,191 B2
- Document ID: US12214191B2
- Status: Granted
- Kind: B2
- Application number: 18/363,442
- Filed: 2023-08-01
- Published: 2024-01-25
- Granted: 2025-02-04
- Earliest claimed: 2020-05-08
- Assignees: Novocure GmbH
- Applicants: Novocure GmbH
- Inventors: Tali Voloshin-Sela
- Web page: https://patentgrep.com/patent/US12214191B2

## CPC

- A61N 1/0492: {Patch electrodes (A61N1/0412, A61N1/0428 take precedence)}
- A61N 1/36: for stimulation
- A61K 31/519: ortho- or peri-condensed with heterocyclic rings
- A61K 31/5377: not condensed and containing further heterocyclic rings, e.g. timolol
- A61K 41/0028: {Disruption, e.g. by heat or ultrasounds, sonophysical or sonochemical activation, e.g. thermosensitive or heat-sensitive liposomes, disruption of calculi with a medicinal preparation and ultrasounds}
- A61N 1/06: for high-frequency therapy
- A61N 1/36002: {Cancer treatment, e.g. tumour}
- A61N 1/36034: {specified by the stimulation parameters}
- A61N 1/406: {using implantable thermoseeds or injected particles for localized hyperthermia (preparations of seeds and particles A61K41/0052)}
- C12N 5/0696: {Artificially induced pluripotent stem cells, e.g. iPS}
- A61N 1/0476: {Array electrodes (including any electrode arrangement with more than one electrode for at least one of the polarities)}
- C12N 2529/00: Culture process characterised by the use of electromagnetic stimulation
- A61N: ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY (measurement of bioelectric currents A61B; surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body A61B18/00; anaesthetic apparatus in general A61M; incandescent lamps H01K; infrared radiators for heating H05B)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Disclosed are methods and compositions for preventing or disrupting mitosis of pluripotent stem cells, killing pluripotent stem cells, preventing or disrupting division of pluripotent stem cells, reducing the viability of pluripotent stem cells, slowing the progression or differentiation of pluripotent stem cells, and treating an ectopic pregnancy.

## Claims

### Claim 1

A method of treating ectopic pregnancy comprising:
distributing an alternating electric field to a target site of a subject for a period of time, the alternating electric field having a frequency and field strength, wherein the frequency is 25 kHz to 1 MHz, wherein the field strength is 0.1 V to 10 V/cm,
wherein the target site comprises an ectopic pregnancy.

### Claim 2 (depends on 1)

The method of claim 1, wherein the target site comprises pluripotent stem cells.

### Claim 3 (depends on 2)

The method of claim 2, wherein the pluripotent stem cells are fetal stem cells, embryonic stem cells or induce pluripotent stem cells.

### Claim 4 (depends on 1)

The method of claim 1, wherein the target site is in a fallopian tube or on a cesarean scar of the subject.

### Claim 5 (depends on 1)

The method of claim 1, further comprising administering gefitinib, methotrexate, or a combination thereof to the subject.

### Claim 6 (depends on 1)

The method of claim 1, wherein the subject has elevated levels of human chorionic gonadotropin (HCG).

### Claim 7 (depends on 1)

The method of claim 1, wherein the subject has been identified to have an abnormal pattern in the rise of levels of human chorionic gonadotropin (HCG).

### Claim 8 (depends on 1)

The method of claim 1, wherein the subject has been diagnosed with an ectopic pregnancy.

### Claim 9 (depends on 8)

The method of claim 8, wherein the subject has been diagnosed via ultrasound.

### Claim 10 (depends on 1)

The method of claim 1, wherein the alternating electric field is a tumor-treating field.

### Claim 11 (depends on 1)

The method of claim 1, wherein one or more arrays of electrodes is present on the skin of the subject, wherein the one or more arrays distributes the alternating electric field.

### Claim 12 (depends on 2)

The method of claim 2, wherein the method does not use heat to kill or ablate the pluripotent stem cells at the target site.

### Claim 13 (depends on 1)

The method of claim 1, wherein the alternating electric field prevents or disrupts mitosis of the pluripotent stem cells at the target site.