# US 12,214,070 B2 · Cosmetic use of engineered postbiotics comprising bacteriocins and/or endolysins > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,214,070 B2 - Document ID: US12214070B2 - Status: Granted - Kind: B2 - Application number: 18/185,696 - Filed: 2023-03-17 - Published: 2023-07-13 - Granted: 2025-02-04 - Earliest claimed: 2021-03-19 - Assignees: Eligo Bioscience - Applicants: Eligo Bioscience - Inventors: Antoine Decrulle; Xavier Duportet - Web page: https://patentgrep.com/patent/US12214070B2 ## CPC - A61K 8/99: from microorganisms other than algae or fungi, e.g. protozoa or bacteria - A61K 35/747: Lactobacilli, e.g. L. acidophilus or L. brevis - A61K 38/164: {from bacteria} - A61K 38/47: acting on glycosyl compounds (3.2), e.g. cellulases, lactases - A61P 17/00: Drugs for dermatological disorders - A61Q 15/00: Anti-perspirants or body deodorants (deodorisation of air A61L9/00) - A61Q 19/005: {Preparations for sensitive skin} - A61Q 19/007: {Preparations for dry skin} - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS ## Abstract A method for the cosmetic caring of the skin and/or mucosa, comprising applying a postbiotic composition comprising at least one postbiotic and at least one bacteriocin and/or endolysin, wherein the postbiotic is preferably a bacterial lysate preferably obtained from bacteria heterologously expressing the at least one bacteriocin and/or endolysin and wherein the postbiotic and the at least one bacteriocin and/or endolysin have a synergistic effect in the cosmetic caring method. ## Claims ### Claim 1 A method for killing unfavorable bacteria on a healthy skin and/or mucosa of a subject, comprising applying on said healthy skin and/or mucosa of said subject an engineered postbiotic composition comprising a microbial lysate and lysostaphin, wherein said microbial lysate and said lysostaphin have a synergistic effect in killing unfavorable bacteria on said healthy skin and/or mucosa of said subject. ### Claim 2 (depends on 1) The method according to claim 1, wherein said microbial lysate is obtained from GRAS and/or probiotic bacteria. ### Claim 3 (depends on 1) The method according to claim 1, wherein said microbial lysate is obtained from bacteria isolated from a subject. ### Claim 4 (depends on 1) The method according to claim 1, wherein said microbial lysate is obtained from Lactobacillus rhamnosus bacteria. ### Claim 5 (depends on 1) The method according to claim 1, wherein said microbial lysate is obtained from bacteria which do not comprise any antibiotic resistance marker. ### Claim 6 (depends on 1) The method according to claim 1, wherein said postbiotic composition comprises at least two different bacteriocins and/or endolysins. ### Claim 7 (depends on 6) The method according to claim 6, wherein said at least two different bacteriocins and/or endolysins target the same bacterial species. ### Claim 8 (depends on 6) The method according to claim 6, wherein said at least two different bacteriocins and/or endolysins target different bacterial species. ### Claim 9 (depends on 1) The method according to claim 1, wherein said engineered postbiotic composition stimulates growth of at least one commensal bacterial species of the subject. ### Claim 10 (depends on 9) The method according to claim 9, wherein said engineered postbiotic composition stimulates growth of at least one commensal bacterial species of the subject and said lysostaphin targets at least one unfavorable bacterial species. ### Claim 11 A method for the care of sensitive, sensitized, fragile and/or weakened skin and/or mucosa, wherein an engineered postbiotic composition is applied on said sensitive, sensitized, fragile and/or weakened skin and/or mucosa, wherein said engineered postbiotic composition comprises a microbial lysate and lysostaphin, wherein said microbial lysate and said lysostaphin have a synergistic effect in killing unfavorable bacteria on said sensitive, sensitized, fragile and/or weakened skin and/or mucosa of said subject. ### Claim 12 (depends on 11) The method according to claim 11, for the care of unaesthetic and/or unpleasant and/or uncomfortable manifestations of sensitive, sensitized, fragile and/or weakened skin and/or mucosa, wherein said engineered postbiotic is applied on the skin and/or mucosa of a subject with unaesthetic and/or unpleasant and/or uncomfortable manifestations of sensitive, sensitized, fragile and/or weakened skin and/or mucosa. ### Claim 13 (depends on 11) The method according to claim 11, wherein the unaesthetic and/or unpleasant and/or uncomfortable manifestations of sensitive, sensitized, fragile and/or weakened skin and/or mucosa are chosen from the feeling of heat or warmth or tension, tingling, stinging, tightness, itching, pruritus, dry patches, erythema, redness and a mixture of these manifestations. ### Claim 14 (depends on 1) The method according to claim 1, for controlling and/or reducing body odor, wherein said engineered postbiotic composition is applied on the skin of the feet or the skin of the armpits. ### Claim 15 (depends on 1) The method according to claim 1, wherein said engineered postbiotic composition is in the form of a cosmetic formulation for topical application.