# US 12,214,038 B2 · Compositions and methods for cancer immunotherapy

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- Number: US 12,214,038 B2
- Document ID: US12214038B2
- Status: Granted
- Kind: B2
- Application number: 15/328,047
- Filed: 2015-07-22
- Published: 2017-08-03
- Granted: 2025-02-04
- Earliest claimed: 2014-07-22
- Assignees: The Trustees of the University of Pennsylvania
- Applicants: The Trustees of the University of Pennsylvania
- Inventors: Andrea Facciabene
- Web page: https://patentgrep.com/patent/US12214038B2

## CPC

- A61K 40/24: Antigen-presenting cells [APC]
- A61K 35/15: Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cells; Myeloid precursor cells; Antigen-presenting cells, e.g. dendritic cells (presenting a specific antigen A61K39/00; therapeutic combinations of antibodies, or fragments thereof, and blood-derived cells A61K39/00)
- A61K 40/19: Dendritic cells
- A61K 40/428: {Undefined tumor antigens, e.g. tumor lysate or antigens targeted by cells isolated from tumor}
- A61P 35/00: Antineoplastic agents
- A61K 2035/122: {for inducing tolerance or supression of immune responses}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The invention provides compositions and methods for cancer immunotherapy. Specifically, the invention provides an antigen-presenting cell, for example, a dendritic cell that comprises a tumor mitochondrial molecule or a tumor mitochondrial molecule derived from tumor mitochondrial protein lysate or a specific mitochondria derived protein of mitochondrial protein lysate as antigen source.

## Claims

### Claim 1

A tumor-specific immunogenic composition comprising: an antigen-presenting cell pulsed with a tumor mitochondrial protein lysate from a renal cancer or a liver cancer, and wherein said antigen-presenting cell is a dendritic cell.

### Claim 2 (depends on 1)

The composition of claim 1, wherein the composition comprises a buffered medium.

### Claim 3 (depends on 1)

The immunogenic composition of claim 1, wherein the dendritic cell is an autologous dendritic cell from a subject having the renal cancer or the liver cancer.

### Claim 4 (depends on 1)

The immunogenic composition of claim 1, wherein the tumor mitochondrial protein lysate is from a renal cancer.

### Claim 5 (depends on 1)

The immunogenic composition of claim 1, wherein the tumor mitochondrial protein lysate is from a liver cancer.

### Claim 6 (depends on 1)

A method for stimulating an anti-tumor immune response in a subject, the method comprising: administering a therapeutically effective amount of the immunogenic composition of claim 1 to said subject.

### Claim 7 (depends on 6)

The method of claim 6, wherein said antigen-presenting cell is a dendritic cell.

### Claim 8 (depends on 6)

The method of claim 6, wherein said tumor antigen is associated with a renal cancer or a liver cancer.

### Claim 9 (depends on 6)

The method of claim 6, wherein the composition comprises a buffered medium.

### Claim 10 (depends on 6)

The method of claim 6, wherein said subject is a human subject.

### Claim 11 (depends on 1)

A method for treating a renal cancer or a liver cancer by immunotherapy in a subject, the method comprising: administering a therapeutically effective amount of the immunogenic composition of claim 1 to said subject.

### Claim 12 (depends on 11)

The method of claim 11, wherein said antigen-presenting cell is a dendritic cell.

### Claim 13 (depends on 11)

The method of claim 11, wherein the composition comprises a buffered medium.

### Claim 14 (depends on 11)

The method of claim 11, wherein said subject is a human subject.