Virus purification
AssigneeValneva SE
InventorsJana Barbero Calzado, Mario Nebenführ, Robert Schlegl, Michael Weber, Jürgen Heindl-Wruss
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Abstract
Described herein are processes for purifying infectious virus particles and uses of protamine in such processes.
Claims14
A method for separating infectious virus particles from non-infectious virus particles comprising precipitating the non-infectious virus particles with protamine, wherein the virus particles are selected from the group consisting of Paramyxoviridae, Orthomyxoviridae, Flaviviridae, Filoviridae, Arenaviridae, Rhabdoviridae, Togaviridae and Coronaviridae, and wherein the virus particles are not Zika virus particles.
A method for purifying infectious virus particles, comprising the steps of
- i) providing a crude harvest (a) comprising infectious virus particles, non-infectious virus particles, and impurities, wherein the impurities are generated from growing said virus particles on a cell substrate;
- ii) contacting said crude harvest (a) with an agent comprising protamine to obtain a virus preparation (b) comprising infectious virus particles, wherein the enrichment of infectious virus particles in the virus preparation (b) relative to total virus particles in the crude harvest (a) is in the range of at least 50% to 95%,
- wherein the virus particles are selected from the group consisting of Paramyxoviridae, Orthomyxoviridae, Flaviviridae, Filoviridae, Arenaviridae, Rhabdoviridae, Togaviridae and Coronaviridae, and wherein the virus particles are not Zika virus particles.
Dependencies are inferred from the claim text.