# US 12,214,027 B2 · Stabilisation of live mollicutes bacteria in a liquid composition

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- Number: US 12,214,027 B2
- Document ID: US12214027B2
- Status: Granted
- Kind: B2
- Application number: 17/441,768
- Filed: 2020-03-27
- Published: 2022-06-09
- Granted: 2025-02-04
- Earliest claimed: 2019-03-29
- Assignees: Intervet Inc.
- Applicants: Intervet inc.
- Inventors: Martin Piest; Edwin Kets; Paul Vermeij; Marian Anna Catharina Jozefina Heijnen; Nöel Dautzenberg
- Web page: https://patentgrep.com/patent/US12214027B2

## CPC

- A61K 39/0241: {Mollicutes, e.g. Mycoplasma, Erysipelothrix}
- A61K 2039/52: {Bacterial cells; Fungal cells; Protozoal cells}
- A61K 2039/552: {Veterinary vaccine}
- A61K 2039/55583: {Polysaccharides}
- A61P 31/04: Antibacterial agents
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention relates to a liquid composition of live Mollicutes bacteria and a stabiliser, whereby the stabiliser is a natural deep-eutectic solvent (NADES). In this liquid composition the live Mollicutes are stabilised without need for freezing or freeze-drying. This allows various advantageous uses in diagnostics and medicine, specifically as a liquid vaccine for use against infection or disease caused by Mollicutes bacteria, for human- or non-human animals.

## Claims

### Claim 1

A liquid composition for protection against infection or disease caused by Mollicutes bacteria, comprising live Mollicutes bacteria and a stabiliser, wherein the stabiliser is a natural deep-eutectic solvent (NADES), wherein the composition has a water activity of 0.85 or less, and a water content of 50% w/w or less.

### Claim 2 (depends on 1)

The liquid composition of claim 1, wherein the NADES comprises an organic salt and a polyol.

### Claim 3 (depends on 1)

The liquid composition of claim 1, wherein the NADES comprises two or more compounds that are all polyols.

### Claim 4 (depends on 2)

The liquid composition of claim 2, wherein the polyol is a sugar or a sugar-alcohol.

### Claim 5 (depends on 4)

The liquid composition of claim 4, wherein the sugar is selected from sucrose and trehalose, or in that the sugar-alcohol is one or more selected from: erythritol, xylitol, and sorbitol.

### Claim 6 (depends on 2)

The liquid composition of claim 2, wherein the organic salt is one or more selected from salts of: betaine, proline, choline, methionine, and arginine.

### Claim 7 (depends on 1)

The liquid composition of claim 1, wherein the Mollicutes bacteria are attenuated in respect of their pathogenic properties.

### Claim 8 (depends on 1)

A method for manufacturing the liquid composition of claim 1, comprising the steps of;
a. providing the NADES, and
b. admixing the NADES with live Mollicutes bacteria, wherein the admixture has a water activity of 0.85 or less, and a water content of 50% w/w or less.

### Claim 9 (depends on 8)

A liquid composition obtainable by the method of claim 8.

### Claim 10 (depends on 1)

A kit of parts comprising at least two containers: one container comprising the liquid composition of claim 1, and one container comprising a liquid diluent.

### Claim 11 (depends on 1)

A method of vaccinating a human or non-human animal target against infection or disease caused by Mollicutes bacteria, comprising administering to said target the liquid composition of claim 1.

### Claim 12 (depends on 10)

A method of vaccinating a human or non-human animal target against infection or disease caused by Mollicutes bacteria, comprising mixing from the kit of parts of claim 10, the contents of the container comprising the liquid composition with the contents of the container comprising a liquid diluent to form a mixture and then administering the mixture to the human or non-human animal target.

### Claim 13 (depends on 9)

A vaccine for protection against infection or disease caused by Mollicutes bacteria, comprising the liquid composition of claim 9.

### Claim 14 (depends on 13)

A method of vaccinating a human or non-human animal target against infection or disease caused by Mollicutes bacteria, comprising administering to the target the liquid vaccine of claim 13.