# US 12,214,009 B2 · Compositions and methods for improving bruising and rejuvenating skin

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 12,214,009 B2
- Document ID: US12214009B2
- Status: Granted
- Kind: B2
- Application number: 18/212,119
- Filed: 2023-06-20
- Published: 2023-11-02
- Granted: 2025-02-04
- Earliest claimed: 2019-05-08
- Assignees: Alastin Skincare, Inc.
- Applicants: Alastin Skincare, Inc.
- Inventors: Alan David Widgerow; John A. Garruto
- Web page: https://patentgrep.com/patent/US12214009B2

## CPC

- A61K 38/06: Tripeptides
- A61Q 19/08: Anti-ageing preparations
- A61K 8/14: Liposomes; Vesicles
- A61K 8/64: Proteins; Peptides; Derivatives or degradation products thereof
- A61K 9/0014: {Skin, i.e. galenical aspects of topical compositions (non-active ingredients are additionally classified in A61K47/00; A61K9/0009, A61K9/0021, A61K9/7015, A61K9/7023 take precedence; cosmetic preparations A61K8/00, A61Q; preparations for wound dressings or bandages A61L26/00)}
- A61K 9/127: Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
- A61K 36/05: Chlorophycota or chlorophyta (green algae), e.g. Chlorella
- A61K 36/07: Basidiomycota, e.g. Cryptococcus
- A61K 36/23: Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K 38/07: Tetrapeptides
- A61K 38/08: Peptides having 5 to 11 amino acids {(A61K38/043 - A61K38/046 take precedence)}
- A61K 38/40: Transferrins, e.g. lactoferrins, ovotransferrins
- A61P 17/00: Drugs for dermatological disorders
- A61P 17/02: for treating wounds, ulcers, burns, scars, keloids, or the like
- A61P 31/04: Antibacterial agents
- A61P 41/00: Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
- A61K 2800/5922: At least two compounds being classified in the same subclass of A61K8/18
- A61K 2800/884: Sequential application
- A61K 2800/91: Injection
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

Compositions and methods for improving bruising, stimulating elastin and/or collagen production, stimulating intrinsic hyaluronic acid production, stimulating adipogenesis, reducing inflammation, or combinations thereof are provided herein. Compositions and methods described herein may be used following a cosmetic procedure.

## Claims

### Claim 1

A method for improving healing of a bruise or improving appearance of a bruise on human skin, the method comprising:
administering a topical composition to human skin that has been subjected to a medical procedure, wherein the topical composition comprises:
a tripeptide-1; and
a hexapeptide-11.

### Claim 2 (depends on 1)

The method of claim 1, wherein the medical procedure is an injection or an invasive surgery.

### Claim 3 (depends on 2)

The method of claim 2, wherein the injection comprises a filler or a neurotoxin.

### Claim 4 (depends on 1)

The method of claim 1, wherein the tripeptide-1 is present at a concentration of 1-10 ppm.

### Claim 5 (depends on 1)

The method of claim 1, wherein the hexapeptide-11 is present at a concentration of 50-150 ppm.

### Claim 6 (depends on 1)

The method of claim 1, wherein the composition further comprises at least one of a hexapeptide-12, a hexapeptide-38, or a tetrapeptide-2.

### Claim 7 (depends on 6)

The method of claim 6, wherein at least one of the hexapeptide-11, the hexapeptide-38, the hexapeptide-12, or the tetrapeptide-2 is encapsulated in a liposome.

### Claim 8 (depends on 7)

The method of claim 7, wherein the liposome comprises at least one of lactoferrin or phosphatidylserine.

### Claim 9 (depends on 1)

The method of claim 1, wherein the composition further comprises at least one of: Ledun palustre extract, dill extract, Tremella fuciformis extract, butylene glycol, glycerin, squalane, Dunaliella salina extract, phospholipids, tocopherol, ascorbyl palrnitate, xanthan gum, betaine, propanediol, lecithin, caprylic/capric triglyceride, caprylyl glycol, caprylyl methicone, phenoxy ethanol, ethylhexylglycerin, polyacrylate-13, polyisobutene, polysorhate 20, caprylhydroxamic acid, disodium EDTA, Arnica Montana extract, sorbitan isostearate, pentylene glycol, glucose, sunflower seed oil, radish root ferment filtrate, potassium sorbate, sodium hyaluronate crosspolyrner, xylilylglucoside, anhydroxyliml, xylitol, hydroxymethoxyphenyl decanone, or combinations thereof.

### Claim 10

A method for improving healing of a bruise or improving appearance of a bruise on human skin, the method comprising:
administering a topical composition to human skin that has been subjected to a cosmetic procedure, wherein the topical composition comprises:
a tripeptide-1; and
a hexapeptide-11.

### Claim 11 (depends on 10)

The method of claim 10, wherein the cosmetic procedure is an injection of a filler, micro-needling, an injection of a neurotoxin, or an invasive surgery.

### Claim 12 (depends on 10)

The method of claim 10, wherein the tripeptide-1 is present at a concentration of 1-10 ppm.

### Claim 13 (depends on 10)

The method of claim 10, wherein the hexapeptide-11 is present at a concentration of 50-150 ppm.

### Claim 14 (depends on 10)

The method of claim 10, wherein the composition further comprises at least one of a hexapeptide-12, a hexapeptide-38, or a tetrapeptide-2.

### Claim 15 (depends on 14)

The method of claim 14, wherein at least one of the hexapeptide-11, the hexapeptide-38, the hexapeptide-12, or the tetrapeptide-2 is present in a liposome.

### Claim 16 (depends on 15)

The method of claim 15, wherein the liposome comprises at least one of lactoferrin or phosphatidylserine.

### Claim 17 (depends on 10)

The method of claim 10, wherein the composition further comprises at least one of: Ledum palustre extract, dill extract, Tremella fuciformis extract, butylene glycol, glycerin, squalane, Dunaliella salina extract, phospholipids, tocopherol, ascorbyl palmitate, xanthan gum, betaine, propanediol, lecithin, caprylic/capric triglyceride, caprylyl glycol, caprylyl nlethicone, phenoxy ethanol, ethylhexylglycerin, polyacrylate-13, polyisobutene, polysorbate 20, caprylhydroxamic acid, disodium EDTA, Arnica Montana extract, sorbitan isostearate, pentylene glycol, glucose, sunflower seed oil, radish root ferment filtrate, potassium sorbate, sodium hyaluronate crosspolymer, xylitylglucoside, anhydroxylitol, xylitol, hydroxymethoxyphenyl decanone, or combinations thereof.

### Claim 18

A method for improving healing of a bruise or improving appearance of a bruise on human skin, the method comprising:
instructing the administration of a topical composition to human skin that has been subjected to a medical or cosmetic procedure, wherein the topical composition comprises:
a tripeptide-1; and
a hexapeptide-11.