# US 12,213,506 B2 · Compositions and methods to counteract processes associated with inflammation and senescence and to support cellular energy and/or metabolism

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- Number: US 12,213,506 B2
- Document ID: US12213506B2
- Status: Granted
- Kind: B2
- Application number: 17/987,747
- Filed: 2022-11-15
- Published: 2023-11-09
- Granted: 2025-02-04
- Earliest claimed: 2021-06-28
- Assignees: DailyColors Health Inc.
- Applicants: DailyColors Health Inc.
- Inventors: Hartley Pond
- Web page: https://patentgrep.com/patent/US12213506B2

## CPC

- A23L 33/105: Plant extracts, their artificial duplicates or their derivatives
- A23L 33/135: Bacteria or derivatives thereof, e.g. probiotics
- A23L 33/15: Vitamins
- A61K 36/185: Magnoliopsida (dicotyledons)
- A61K 36/23: Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K 36/45: Ericaceae or Vacciniaceae (Heath or Blueberry family), e.g. blueberry, cranberry or bilberry
- A61K 36/53: Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K 36/63: Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
- A61K 36/73: Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K 36/742: {Coffea, e.g. coffee}
- A61K 36/752: Citrus, e.g. lime, orange or lemon
- A61K 36/81: Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K 36/87: Vitaceae or Ampelidaceae (Vine or Grape family), e.g. wine grapes, muscadine or peppervine
- A61K 36/8962: Allium, e.g. garden onion, leek, garlic or chives
- A61K 36/9068: Zingiber, e.g. garden ginger
- A23L 33/40: {Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula}
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

Contemplated compositions and methods are based on a combination of polyphenols commonly found in the Mediterranean diet that, when combined, reduce pro-inflammatory signaling and expression of senescence-associated genes and support mitochondrial biogenesis and cellular energy metabolism. Notably, the observed pleiotropic effects did not only span across multiple signaling pathways, but also exhibited synergistic activity with respect to a number of markers associated with reduction of age-related decline in energy, immunity, and increase or persistence of chronic subacute inflammation. Viewed from a different perspective, contemplated compositions represent a symphony of biochemically diverse molecules that form the foundation for numerous benefits typically observed with the Mediterranean diet.

## Claims

### Claim 1

A method of supplementing human nutrition, comprising:
administering to a human a nutritional supplement that consists of:
a nutritionally acceptable carrier;
a plurality of polyphenol-containing plant materials consisting of: red color, green color, orange-yellow color, and purple-blue color;
wherein the red colored plant materials, based on a total weight of the nutritional supplement, consist of:
about 10 wt. % of an apple extract,
about 10 wt. % of a pomegranate extract,
about 1.85 wt. % of a tomato powder, and
about 2.45 wt. % of a beet root powder,
wherein the green colored plant materials, based on a total weight of the nutritional supplement, consist of:
about 6.38 wt. % of an olive extract,
about 7.5 wt. % of a rosemary extract,
about 7.5 wt. % of a green coffee bean extract, and
about 2.5 wt. % of a kale powder,
wherein the orange-yellow colored plant materials, based on a total weight of the nutritional supplement, consist of:
about 10 wt. % of an onion extract,
about 10 wt. % of a ginger extract,
about 2.5 wt. % of a grapefruit extract, and
about 2.45 wt. % of a carrot powder,
wherein the purple-blue colored plant materials, based on a total weight of the nutritional supplement, consist of:
about 15.75 wt. % of a grape extract,
about 2.5 wt. % of a blueberry extract,
about 2.43 wt. % of a black currant powder, and
about 2.43 wt. % of an elderberry powder;
about 3.19 wt. % of maltodextrin;
about 0.31 wt. % of corn starch;
about 0.25 wt. % of slica; and
about 0.03 wt. % of sunflower lecithin;
wherein the nutritionally acceptable carrier is present in an amount to balance the nutritional supplement to 100 wt. %;
wherein the plant materials and/or polyphenols contained therein, when tested in vitro, have at least one of the following effects:
(a) increase ATP levels in RAW 264.1 mouse macrophages at a dosage of 1 μg/mL,
(b) increase glucose uptake into mouse C2C12 muscle cells at a dosage of 10 μg/mL, and
(c) increase mitochondrial biogenesis in Neuro-2a cells at a dosage of 10 μg/mL.

### Claim 2 (depends on 1)

The method of claim 1, wherein the plurality of plant materials and/or polyphenols contained therein, are provided in a fresh, a dried, and/or an extracted form.

### Claim 3 (depends on 1)

The method of claim 1, wherein the plurality of plant materials and/or polyphenols contained therein, are formulated in a bulk powder for oral administration.

### Claim 4 (depends on 1)

The method of claim 1, wherein the plurality of plant materials and/or polyphenols contained therein, are provided as a whole plant or as a portion of a whole plant.

### Claim 5 (depends on 1)

The method of claim 1, wherein the plurality of plant materials and/or polyphenols contained therein, are formulated in a dosage unit for oral administration.

### Claim 6 (depends on 5)

The method of claim 5, wherein the dosage unit is formulated as a capsule, a gummy, a powder, a liquid, or a gel.

### Claim 7 (depends on 1)

The method of claim 1, wherein the increased glucose uptake into the muscle cells is similar or greater than an increased glucose uptake using rosiglitazone.

### Claim 8 (depends on 1)

The method of claim 1, wherein administering the nutritional supplement occurs over a period of at least 30 days.

### Claim 9 (depends on 1)

The method of claim 1, wherein the nutritional supplement further comprises an additional ingredient to impart an additional functionality.

### Claim 10 (depends on 1)

The method of claim 1, wherein the mitochondrial biogenesis is similar or greater than an increased mitochondrial biogenesis using a rosiglitazone.

### Claim 11 (depends on 5)

The method of claim 5, wherein the dosage unit contains between 50 and 1,000 mg of the plurality of plant materials and/or polyphenols contained therein.

### Claim 12

A method of supplementing human nutrition, comprising:
administering to a human a nutritional supplement that consists of:
a nutritionally acceptable carrier;
a plurality of polyphenol-containing plant materials consisting of: red color, green color, orange-yellow color, and purple-blue color;
wherein the red colored plant materials, based on a total weight of the nutritional supplement, consist of:
about 10 wt. % of an apple extract,
about 10 wt. % of a pomegranate extract,
about 1.85 wt. % of a tomato powder, and
about 2.45 wt. % of a beet root powder,
wherein the green colored plant materials, based on a total weight of the nutritional supplement, consist of:
about 6.38 wt. % of an olive extract,
about 7.5 wt. % of a rosemary extract,
about 7.5 wt. % of a green coffee bean extract, and
about 2.5 wt. % of a kale powder,
wherein the orange-yellow colored plant materials, based on a total weight of the nutritional supplement, consist of:
about 10 wt. % of an onion extract,
about 10 wt. % of a ginger extract,
about 2.5 wt. % of a grapefruit extract, and
about 2.45 wt. % of a carrot powder,
wherein the purple-blue colored plant materials, based on a total weight of the nutritional supplement, consist of:
about 15.75 wt. % of a grape extract,
about 2.5 wt. % of a blueberry extract,
about 2.43 wt. % of a black currant powder, and
about 2.43 wt. % of an elderberry powder;
about 3.19 wt. % of maltodextrin;
about 0.31 wt. % of corn starch;
about 0.25 wt. % of slica; and
about 0.03 wt. % of sunflower lecithin;
wherein the nutritionally acceptable carrier is present in an amount to balance the nutritional supplement to 100 wt. %;
wherein the plant materials and/or polyphenols contained therein, when tested in vitro, reduces
(a) at least one pro-inflammatory cytokine in human primary monocytes at a dosage of at least 10 μg/mL,
(b) NFiB signaling in HEK293T cells at a dosage of at least 50 μg/mL, and/or
(c) at least one pro-inflammatory adipokine in mouse 3T3-L1 cells at a dosage of at least 10 μg/mL.

### Claim 13 (depends on 12)

The method of claim 12, wherein the plurality of plant materials and/or polyphenols contained therein are formulated in a single dosage unit or bulk powder for oral administration.

### Claim 14 (depends on 13)

The method of claim 13, wherein the dosage unit contains between 50 and 1,000 mg of the plurality of plant materials and/or polyphenols contained therein.

### Claim 15

A method of supplementing human nutrition, comprising:
administering to a human a nutritional supplement comprising:
a nutritionally acceptable carrier; in combination with
a plurality of polyphenol-containing plant materials comprises: red color, green color, orange-yellow color, and purple-blue color;
wherein the red colored plant materials, based on a total weight of the nutritional supplement, comprise:
about 10 wt. % of an apple extract,
about 10 wt. % of a pomegranate extract,
about 1.85 wt. % of a tomato powder, and
about 2.45 wt. % of a beet root powder,
wherein the green colored plant materials, based on a total weight of the nutritional supplement, comprise:
about 6.38 wt. % of an olive extract,
about 7.5 wt. % of a rosemary extract,
about 7.5 wt. % of a green coffee bean extract, and
about 2.5 wt. % of a kale powder,
wherein the orange-yellow colored plant materials, based on a total weight of the nutritional supplement, comprise:
about 10 wt. % of an onion extract,
about 10 wt. % of a ginger extract,
about 2.5 wt. % of a grapefruit extract, and
about 2.45 wt. % of a carrot powder,
wherein the purple-blue colored plant materials, based on a total weight of the nutritional supplement, comprise:
about 15.75 wt. % of a grape extract,
about 2.5 wt. % of a blueberry extract,
about 2.43 wt. % of a black currant powder, and
about 2.43 wt. % of an elderberry powder.

### Claim 16

A method of supplementing human nutrition, comprising:
administering to a human a nutritional supplement consisting of:
a nutritionally acceptable carrier; in combination with
a plurality of polyphenol-containing plant materials comprises: red color, green color, orange-yellow color, and purple-blue color;
wherein the red colored plant materials, based on a total weight of the nutritional supplement, consist of:
about 10 wt. % of an apple extract,
about 10 wt. % of a pomegranate extract,
about 1.85 wt. % of a tomato powder, and
about 2.45 wt. % of a beet root powder,
wherein the green colored plant materials, based on a total weight of the nutritional supplement, consist of:
about 6.38 wt. % of an olive extract,
about 7.5 wt. % of a rosemary extract,
about 7.5 wt. % of a green coffee bean extract, and
about 2.5 wt. % of a kale powder,
wherein the orange-yellow colored plant materials, based on a total weight of the nutritional supplement, consist of:
about 10 wt. % of an onion extract,
about 10 wt. % of a ginger extract,
about 2.5 wt. % of a grapefruit extract, and
about 2.45 wt. % of a carrot powder,
wherein the purple-blue colored plant materials, based on a total weight of the nutritional supplement, consist of:
about 15.75 wt. % of a grape extract,
about 2.5 wt. % of a blueberry extract,
about 2.43 wt. % of a black currant powder, and
about 2.43 wt. % of an elderberry powder;
wherein the nutritionally acceptable carrier is present in an amount to balance the nutritional supplement to 100 wt. %.

### Claim 17

A nutritional supplement comprising:
a nutritionally acceptable carrier; in combination with
a plurality of polyphenol-containing plant materials comprises: red color, green color, orange-yellow color, and purple-blue color;
wherein the red colored plant materials, based on a total weight of the nutritional supplement, comprise:
about 10 wt. % of an apple extract,
about 10 wt. % of a pomegranate extract,
about 1.85 wt. % of a tomato powder, and
about 2.45 wt. % of a beet root powder,
wherein the green colored plant materials, based on a total weight of the nutritional supplement, comprise:
about 6.38 wt. % of an olive extract,
about 7.5 wt. % of a rosemary extract,
about 7.5 wt. % of a green coffee bean extract, and
about 2.5 wt. % of a kale powder,
wherein the orange-yellow colored plant materials, based on a total weight of the nutritional supplement, comprise:
about 10 wt. % of an onion extract,
about 10 wt. % of a ginger extract,
about 2.5 wt. % of a grapefruit extract, and
about 2.45 wt. % of a carrot powder,
wherein the purple-blue colored plant materials, based on a total weight of the nutritional supplement, comprise:
about 15.75 wt. % of a grape extract,
about 2.5 wt. % of a blueberry extract,
about 2.43 wt. % of a black currant powder, and
about 2.43 wt. % of an elderberry powder.

### Claim 18

A nutritional supplement consisting of:
a nutritionally acceptable carrier; in combination with
a plurality of polyphenol-containing plant materials comprises: red color, green color, orange-yellow color, and purple-blue color;
wherein the red colored plant materials, based on a total weight of the nutritional supplement, consist of:
about 10 wt. % of an apple extract,
about 10 wt. % of a pomegranate extract,
about 1.85 wt. % of a tomato powder, and
about 2.45 wt. % of a beet root powder,
wherein the green colored plant materials, based on a total weight of the nutritional supplement, consist of:
about 6.38 wt. % of an olive extract,
about 7.5 wt. % of a rosemary extract,
about 7.5 wt. % of a green coffee bean extract, and
about 2.5 wt. % of a kale powder,
wherein the orange-yellow colored plant materials, based on a total weight of the nutritional supplement, consist of:
about 10 wt. % of an onion extract,
about 10 wt. % of a ginger extract,
about 2.5 wt. % of a grapefruit extract, and
about 2.45 wt. % of a carrot powder,
wherein the purple-blue colored plant materials, based on a total weight of the nutritional supplement, consist of:
about 15.75 wt. % of a grape extract,
about 2.5 wt. % of a blueberry extract,
about 2.43 wt. % of a black currant powder, and
about 2.43 wt. % of an elderberry powder;
wherein the nutritionally acceptable carrier is present in an amount to balance the nutritional supplement to 100 wt. %.