# US 12,213,503 B2 · Method of treating plant and method of making plant-based food or drink product

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- Number: US 12,213,503 B2
- Document ID: US12213503B2
- Status: Granted
- Kind: B2
- Application number: 17/393,709
- Filed: 2021-08-04
- Published: 2022-02-10
- Granted: 2025-02-04
- Earliest claimed: 2020-08-05
- Assignees: Nichia Corporation
- Applicants: Nichia Corporation
- Inventors: Tomohiro Tsurumoto; Yasuo Fujikawa; Atsushi Okazawa
- Web page: https://patentgrep.com/patent/US12213503B2

## CPC

- A23B 2/53: with ultraviolet light
- A01G 7/045: {with electric lighting}
- A23B 7/015: Preserving by irradiation or electric treatment without heating effect
- A23B 70/50: by irradiation or electric treatment, without heating
- A23L 2/04: Extraction of juices (machines or apparatus for extracting juice A23N1/00, A47J19/00)
- A23L 5/30: Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation (cooking A23L5/10)
- A23L 19/09: {Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks (A23L19/01, A23L19/10, A23L27/63 and A23L21/00 take precedence)}
- C12G 1/00: Preparation of wine or sparkling wine
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A23B: PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A01G: HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING (picking of fruits, vegetables, hops or the like A01D46/00; propagating unicellular algae C12N1/12)
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- C12G: WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES (beer C12C); PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

The present disclosure relates to a method of increasing an amount of a stilbenoid, and/or one or more compounds selected from the group consisting of TCA cycle metabolites, polyamine alkaloids, 4-aminobutyric acid, abscisic acid and salts thereof in a plant, the method comprising steps of: irradiating the plant, part, crushed material or cultured plant cell with light, wherein a fluence at a wavelength range of 275-295 nm is 50,000-2,500,000 μmol/m2, while at the same time a fluence at a wavelength range of 200-270 nm is less than 20% of the fluence at the wavelength range of 275-295 nm; and storing the irradiated plant, part, crushed material or cultured plant cell in a dark place for 1 day or more.

## Claims

### Claim 1

A method of increasing an amount of one or more compounds selected from the group consisting of stilbenoids, TCA cycle metabolites, polyamine alkaloids, 4-aminobutyric acid, abscisic acid and salts in a plant cell culture, the method comprising steps of:
irradiating the plant cell culture with light at a wavelength range of 275-295 nm, at a fluence of 50,000-2,500,000 μmol/m2, while at the same time a fluence at a wavelength range of 200-270 nm is less than 20% of the fluence at the wavelength range of 275-295 nm; and then
storing the irradiated plant cell culture for 1 day or more in a dark place,
wherein the plant cell culture is a callus cell culture.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the irradiating is carried out at a photon flux density at the wavelength range of 275-295 nm of 0.1-600 μmol/m2/s.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the irradiating is carried out with light having a wavelength spectrum with a peak wavelength at 285±5 nm and a full width at half maximum of 5-15 nm.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the cultured plant cell is stored in the dark place at a temperature of 15-25° C.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the cultured plant cell is stored in the dark place for 48 hours or more.

### Claim 6 (depends on 1)

The method according to claim 1, wherein the fluence at the wavelength range of 275-295 nm is 100,000-2,500,000 μmol/m2.

### Claim 7 (depends on 1)

The method according to claim 1, wherein the fluence at the wavelength range of 275-295 nm is 150,000-2,500,000 μmol/m2.

### Claim 8 (depends on 1)

The method according to claim 1, wherein the irradiating is carried out with intermittent light having a duty ratio of 50% or less.

### Claim 9 (depends on 1)

The method according to claim 1, wherein the callus cell culture is red.

### Claim 10 (depends on 9)

The method according to claim 9, wherein the red callus cell culture is from a tissue of grape belonging to the species Vitis vinifera, or an interspecific hybrid between Vitis vinifera and one or more species selected from the group consisting of Vitis labrusca, Vitis amurensis, Vitis mustangensis and Vitis riparia.

### Claim 11 (depends on 10)

The method according to claim 10, wherein the compound is resveratrol.

### Claim 12 (depends on 10)

The method according to claim 10, wherein the callus cell culture is red.

### Claim 13 (depends on 1)

A method of obtaining or extracting one or more compounds selected from the group consisting of stilbenoids, TCA cycle metabolites, polyamine alkaloids, 4-aminobutyric acid, abscisic acid and salts from cultured plant cells in a callus cell culture, the method comprising steps of:
subjecting the cultured plant cells to the irradiation method according to claim 1, and then
obtaining or extracting the compounds from the irradiated cultured plant cells.