# US 12,213,465 B2 · Red transgenic fluorescent ornamental fish

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- Number: US 12,213,465 B2
- Document ID: US12213465B2
- Status: Granted
- Kind: B2
- Application number: 17/528,936
- Filed: 2021-11-17
- Published: 2022-03-10
- Granted: 2025-02-04
- Earliest claimed: 2016-03-11
- Applicants: GloFish, LLC
- Inventors: Alan Blake; Richard Crockett; Aidas Nasevicius
- Web page: https://patentgrep.com/patent/US12213465B2

## CPC

- A01K 67/0275: Genetically modified vertebrates, e.g. transgenic
- A01K 67/02: Breeding vertebrates
- C07K 14/43595: {from coelenteratae, e.g. medusae}
- A01K 2217/052: inducing gain of function
- A01K 2227/40: Fish
- A01K 2267/02: Animal zootechnically ameliorated
- A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present invention relates to transgenic red ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

## Claims

### Claim 1

A transgenic barb progeny whose genome is obtained from a first transgenic barb, wherein the first transgenic barb exhibits fluorescence and comprises a chromosomally integrated expression cassette encoding a fluorescent protein and a second barb, wherein the first transgenic barb exhibits fluorescence and wherein the chromosomally integrated expression cassette in the first transgenic barb is comprised by the “Red barb 1 transformation event,” sperm comprising the Red barb 1 transformation event having been deposited as ECACC accession no. 16121901, wherein the transgenic barb progeny exhibits fluorescence.

### Claim 2 (depends on 1)

The transgenic barb progeny of claim 1, wherein the first barb is heterozygous for the “Red barb 1 transformation event”.

### Claim 3 (depends on 1)

The transgenic barb progeny of claim 1 wherein the first barb is homozygous for the “Red barb 1 transformation event”.

### Claim 4 (depends on 1)

The transgenic barb progeny of claim 1, wherein the second barb is a transgenic barb that exhibits fluorescence.

### Claim 5 (depends on 4)

The transgenic barb progeny of claim 4, wherein the second barb comprises a chromosomally integrated expression cassette encoding a fluorescent protein, the expression cassette comprised by the “Red barb 1 transformation event”, sperm comprising the “Red barb 1 transformation event” having been deposited as ECACC accession no. 1612901.

### Claim 6 (depends on 5)

The transgenic barb progeny of claim 5, wherein the second barb is homozygous for the “Red barb 1 transformation event”.

### Claim 7 (depends on 5)

The transgenic barb progeny of claim 5, wherein the second barb is heterozygous for the “Red barb 1 transformation event”.