# US 12,213,445 B2 · Tomato hybrid DRTJ4056 and parents thereof

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- Number: US 12,213,445 B2
- Document ID: US12213445B2
- Status: Granted
- Kind: B2
- Application number: 17/891,688
- Filed: 2022-08-19
- Published: 2023-04-13
- Granted: 2025-02-04
- Earliest claimed: 2021-10-11
- Assignees: Seminis Vegetable Seeds, Inc.
- Applicants: Seminis Vegetable Seeds, Inc.
- Inventors: Bernardus van den Bosch
- Web page: https://patentgrep.com/patent/US12213445B2

## CPC

- A01H 6/825: {Solanum lycopersicum [tomato]}
- A01H 1/02: Methods or apparatus for hybridisation; Artificial pollination {; Fertility}
- A01H 5/08: Fruits
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

The invention provides seeds and plants of tomato hybrid DRTJ4056, tomato line FIS-XM17-4473, and tomato line FIR-XM17-4474. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of tomato hybrid DRTJ4056, tomato line FIS-XM17-4473, and tomato line FIR-XM17-4474 and to methods for producing a tomato plant produced by crossing such plants with themselves or with another plant, such as a tomato plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of tomato hybrid DRTJ4056, tomato line FIS-XM17-4473, and tomato line FIR-XM17-4474 comprising introduced beneficial or desirable traits.

## Claims

### Claim 1

A tomato plant comprising at least a first set of the chromosomes of tomato line FIS-XM17-4473 or tomato line FIR-XM17-4474, a sample of seed of said lines having been deposited under NCMA Accession No. 202109081 and NCMA Accession No. 202109082, respectively.

### Claim 2 (depends on 1)

A tomato seed that produces the plant of claim 1.

### Claim 3 (depends on 1)

The plant of claim 1, wherein the plant is a plant of said tomato line FIS-XM17-4473 or tomato line FIR-XM17-4474.

### Claim 4 (depends on 1)

The plant of claim 1, wherein the plant is a plant of tomato hybrid DRTJ4056, wherein said plant comprises a first set of chromosomes and a second set of chromosomes, wherein said first set of chromosomes is a set of the chromosomes of tomato line FIS-XM17-4473 and said second set of chromosomes is a set of the chromosomes of tomato line FIR-XM17-4474, a sample of seed of said lines having been deposited under NCMA Accession No. 202109081 and NCMA Accession No. 202109082, respectively.

### Claim 5 (depends on 2)

The seed of claim 2, wherein the seed is a seed of said tomato line FIS-XM17-4473 or tomato line FIR-XM17-4474.

### Claim 6 (depends on 4)

A tomato seed that produces the plant of claim 4.

### Claim 7 (depends on 1)

A plant part of the plant of claim 1, wherein the plant part comprises a cell of said plant.

### Claim 8 (depends on 4)

A tomato plant having all of the physiological and morphological characteristics of the plant of claim 4.

### Claim 9 (depends on 1)

A tissue culture of regenerable cells of the plant of claim 1.

### Claim 10 (depends on 1)

A method of vegetatively propagating the plant of claim 1, the method comprising the steps of:
(a) collecting tissue capable of being propagated from the plant; and
(b) propagating a tomato plant from said tissue.

### Claim 11 (depends on 3)

A method of introducing a trait into a tomato line, the method comprising:
(a) crossing the plant of claim 3 with a donor plant that comprises a trait to produce F1 progeny;
(b) selecting an F1 progeny that comprises the trait;
(c) backcrossing the selected F1 progeny with a plant of a recurrent parent tomato line to produce backcross progeny, wherein said recurrent parent tomato line is tomato line FIS-XM17-4473 or tomato line FIR-XM17-4474, and wherein the plant of the recurrent parent tomato line is of the same line used in the crossing in step (a);
(d) selecting a backcross progeny comprising the trait and otherwise comprising the morphological and physiological characteristics of the recurrent parent tomato line used in step (b); and
(e) repeating steps (c) and (d) three or more times to produce a selected fourth or higher backcross progeny.

### Claim 12 (depends on 11)

A tomato plant produced by the method of claim 11, wherein said plant comprises the trait and otherwise comprises all of the morphological and physiological characteristics of tomato line FIS-XM17-4473 or tomato line FIR-XM17-4474.

### Claim 13 (depends on 1)

A method of producing a tomato plant comprising an added trait, the method comprising introducing a transgene conferring the trait into the plant of claim 1.

### Claim 14 (depends on 13)

A tomato plant produced by the method of claim 13, wherein said plant comprises the trait and otherwise comprises all of the morphological and physiological characteristics of tomato hybrid DRTJ4056, tomato line FIS-XM17-4473, or tomato line FIR-XM17-4474, wherein said tomato hybrid DRTJ4056 comprises a first set of chromosomes and a second set of chromosomes, wherein said first set of chromosomes is a set of the chromosomes of tomato line FIS-XM17-4473 and said second set of chromosomes is a set of the chromosomes of tomato line FIR-XM17-4474, a sample of seed of said lines having been deposited under NCMA Accession No. 202109081 and NCMA Accession No. 202109082, respectively.

### Claim 15

A tomato plant comprising at least a first set of the chromosomes of tomato line FIS-XM17-4473 or tomato line FIR-XM17-4474, a sample of seed of said lines having been deposited under NCMA Accession No. 202109081 and NCMA Accession No. 202109082, respectively, wherein said first set of chromosomes further comprises a transgene.

### Claim 16 (depends on 15)

The plant of claim 15, wherein the transgene confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, environmental stress tolerance, modified carbohydrate metabolism, and modified protein metabolism.

### Claim 17

A single locus converted tomato plant comprising at least a first set of the chromosomes of tomato line FIS-XM17-4473 or tomato line FIR-XM17-4474, a sample of seed of said lines having been deposited under NCMA Accession No. 202109081 and NCMA Accession No. 202109082, respectively, wherein said first set of chromosomes further comprises a single locus conversion.

### Claim 18 (depends on 17)

The plant of claim 17, wherein the single locus conversion confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, environmental stress tolerance, modified carbohydrate metabolism, and modified protein metabolism.

### Claim 19 (depends on 1)

A method for producing a seed of a tomato plant, the method comprising the steps of:
(a) crossing the plant of claim 1 with itself or a different tomato plant; and
(b) allowing the seed to form.

### Claim 20 (depends on 1)

A method of producing a seed of a derived tomato plant, the method comprising the steps of:
(a) producing a derived tomato plant from a seed produced by crossing the plant of claim 1 with itself or a different tomato plant; and
(b) crossing the derived tomato plant with itself or a different tomato plant to obtain a seed of a further derived tomato plant.

### Claim 21 (depends on 20)

The method of claim 20, the method further comprising repeating said producing and crossing steps of (a) and (b) using the seed from said step (b) for producing the tomato plant according to step (a) for at least one generation to produce a seed of an additional derived tomato plant.

### Claim 22 (depends on 1)

A method of producing a tomato fruit, the method comprising:
(a) obtaining the plant of claim 1, wherein the plant has been cultivated to maturity; and
(b) collecting a tomato fruit from the plant.

### Claim 23 (depends on 1)

The tomato plant of claim 1, wherein the plant is a hybrid tomato plant.

### Claim 24 (depends on 23)

A seed that produces the plant of claim 23.
