Kit and method
AssigneeDenka Company Limited
InventorsYasuki Ito, Noriyuki Satoh
Life of this patent
Estimating the term…
Abstract
A kit used for fractionation of small dense LDL cholesterol (sdLDL-C) in a sample, including: a first reagent composition having one or two or more activities selected from the group consisting of cholesterol esterase activity, cholesterol oxidase activity, and sphingomyelinase activity; and a second reagent composition for quantifying the sdLDL-C, in which in an absorption spectrum after storing the first reagent composition at 37° C. for 2 weeks, a ratio R1 represented by ABS400/ABS450 is 0.90 or more and 3.00 or less, and in an absorption spectrum after storing the second reagent composition at 37° C. for 2 weeks, a ratio R1 represented by ABS400/ABS450 is 0.90 or more and 8.00 or less.
Claims12
A kit used for fractionation of small dense LDL cholesterol (sdLDL-C) in a sample, comprising:
- a first reagent composition having one or two or more activities selected from the group consisting of cholesterol esterase activity, cholesterol oxidase activity, and sphingomyelinase activity;
- and
- a second reagent composition for quantifying the sdLDL-C,
- wherein in an absorption spectrum after storing the first reagent composition at 37° C. for 2 weeks, when an absorbance at a wavelength of 400 nm is ABS400, and an absorbance at a wavelength of 450 nm is ABS450, a ratio R1 represented by ABS400/ABS450 is 0.90 or more and 3.00 or less,
- wherein in an absorption spectrum after storing the second reagent composition at 37° C. for 2 weeks, when an absorbance at a wavelength of 400 nm is ABS400 and an absorbance at a wavelength of 450 nm is ABS450, a ratio R1 represented by ABS400/ABS450 is 0.90 or more and 8.00 or less,
- wherein the first reagent composition satisfies one or two conditions of Conditions 1 to 3, and
- wherein the second reagent composition does not satisfy the one or two conditions of Conditions 1 to 3 and satisfies all other conditions,
- wherein Condition 1=comprises a coupler;
- wherein Condition 2=comprises an iron complex;
- and
- wherein Condition 3=possesses a peroxidase activity.
A method of quantifying small dense LDL cholesterol (sdLDL-C) in a sample, comprising:
- (a) allowing a first reagent composition having one or two or more activities selected from the group consisting of cholesterol esterase activity, cholesterol oxidase activity, and sphingomyelinase activity to act on the sample;
- and
- (b) allowing a second reagent composition for quantifying the sdLDL-C to act to quantify cholesterol in a remaining lipoprotein,
- wherein in an absorption spectrum after storing the first reagent composition at 37° C. for 2 weeks, when an absorbance at a wavelength of 400 nm is ABS400, and an absorbance at a wavelength of 450 nm is ABS450, a ratio R1 represented by ABS400/ABS450 is 0.90 or more and 3.00 or less,
- wherein in an absorption spectrum after storing the second reagent composition at 37° C. for 2 weeks, when an absorbance at a wavelength of 400 nm is ABS400 and an absorbance at a wavelength of 450 nm is ABS450, a ratio R1 represented by ABS400/ABS450 is 0.90 or more and 8.00 or less,
- wherein the first reagent composition satisfies one or two conditions of Conditions 1 to 3, and
- wherein the second reagent composition does not satisfy the one or two conditions of Conditions 1 to 3 and satisfies all other conditions,
- wherein Condition 1=comprises a coupler,
- wherein Condition 2=comprises an iron complex;
- and
- wherein Condition 3=possesses a peroxidase activity.
Dependencies are inferred from the claim text.