Engineered Herpes Simplex Virus-1 (HSV-1) vectors and uses thereof
AssigneeMassachusetts Institute of Technology
InventorsRon Weiss, Maria Hottelet Foley, Jin Huh, Ross D. Jones
Life of this patent
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Abstract
Provided herein are engineered HSV-1 vectors comprising a modified HSV-1 genome. The engineered HSV-1 vectors can be used to deliver genetic circuits (e.g., up to 100 kb) to cells in vitro or in vivo. Methods of treating or diagnosing a disease (e.g., cancer) using the engineered HSV-1 vectors described herein are also provided.
Claims19
An engineered Herpes Simplex Virus-1 (HSV-1) vector comprising a modified HSV-1 genome comprising non-functional deletions in both copies of the gene encoding Infected Cell Protein 4 (ICP4), non-functional deletions in both copies of the gene encoding Infected Cell Protein 0 (ICP0), a deletion in the gene encoding Virion Protein 16 (VP16) that results in a truncation of the VP16 protein at amino acid 422, and one or more deletions in one copy of the Latency Associated Transcript (LAT) region,
- wherein a transcript of a LAT region is produced in an infected cell, and
- wherein the HSV-1 genome comprises a nucleotide sequence having 95% sequence identity to the nucleotide sequence of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.