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US 12,209,236 B2Granted
Overexpression of fumarate reductase results in an increased fermentation rate in yeast
AssigneeDanisco US Inc.
InventorCelia Emily Gaby Payen
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Abstract
Described are compositions and methods relating to modified yeast that overexpress fumarate reductase. The yeast cells have an increased fermentation rate compared to their parental cells. Such yeast cells ae particularly useful for large-scale ethanol production from starch substrates where an increased rate of ethanol production is desirable.
Claims19
A method for increasing the rate of ethanol production in yeast cells grown on a carbohydrate substrate comprising:
- introducing into parental yeast cells a genetic alteration that causes the modified yeast cells to produce an increased amount of fumarate reductase compared to the parental yeast cells,
- wherein the modified yeast cells have an increased rate of fermentation compared to the rate of fermentation of the parental yeast cells under equivalent fermentation conditions;
- wherein the carbohydrate substrate is substantially free of xylose;
- and
- wherein the fumarate reductase comprises an amino acid sequence having at least 90% sequence identity to the sequence set forth by SEQ ID NO:
- 3 or an amino acid sequence having at least 90% sequence identity to amino acids 33 to 501 of the sequence set forth by SEQ ID NO:3.
3 more elements
Dependencies are inferred from the claim text.