Systems and methods for fabricating microfluidic devices

A device comprising:

• a construction comprising one or more channels having a resolution of dimensions higher than a threshold of less than 100, 150, 200 or 250 microns;
• wherein the device is constructed using an additive manufacturing technique selected to provide the resolution of dimensions of the one or more channels higher than the threshold of less than 100, 150, 200 or 250 microns;
• wherein the device is constructed by sequentially applying the additive manufacturing technique to a material to create a plurality of layers of the device, based on a model for the device specifying a plurality of parameters;
• wherein the material used to construct the device provides for non-cytotoxicity over an extended period of time, optical transparency to visible light, reduced auto-fluorescence to enable data capture of the device operation via fluorescent images, and fabrication to reproduce features of less than 100 microns;
• and
• wherein the material includes at least 50%, or 60% or 70% by weight of polyethyl methacrylate polymer or copolymer.