Method of joining sterile connectors

A method of connecting a first tubing to a second tubing for aseptic fluid transfer, comprising:

• providing two holders each with a channel designed to house the first or second tubing and each with a mating surface at one end of the channel;
• attaching a male connector having a central bore and a tapered distal end to a female connector having a central bore that is an elastic material designed to surround the male connector and form a seal, wherein the male connector has the tapered distal end furthest from the first tubing when the first tubing is connected to a proximal end of the male connector and the female connector has a first end furthest from the second tubing when a second end of the female connector is connected with the second tubing;
• placing the first tubing in the channel in the first holder and the second tubing in the channel in the second holder such the respective male and female connectors are opposing each other;
• attaching a first tape with adhesive on one surface to a portion of the first tubing, wherein the first tape has sufficient length to extend past the distal end of the male connector and has no adhesive on the surfaces which extend from the distal end of the male connector to an end of the first tape;
• attaching the second tape with adhesive on one side to a portion of the second tubing, wherein the second tape has sufficient length to extend past the first end of the female connector and has no adhesive on the surfaces which extend from the end of the female connector to an end of the second tape;
• and
• placing the mating surfaces of the holders adjacent and pulling the ends of the first tape and the second tape such that the male connector and the female connector advance towards each other, and the male connector is pulled inside the female connector and forms the seal and provides a fluid connection from the first tubing to the second tubing.